Droplet-based microfluidic lab-on-a-chip for glucose detection

被引：317

作者：

Srinivasan, V ^{[1
]}

Pamula, VK ^{[1
]}

Fair, RB ^{[1
]}

机构：

[1] Duke Univ, Dept Elect Engn, Durham, NC 27708 USA

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 507卷 / 01期

关键词：

electrowetting; droplet; glucose; lab-on-a-chip; microfluidics;

D O I：

10.1016/j.aca.2003.12.030

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A microfluidic lab-on-a-chip (LoC) platform for in vitro measurement of glucose for clinical diagnostic applications is presented in this paper. The LoC uses a discrete droplet format in contrast to conventional continuous flow microfluidic systems. The droplets act as solution-phase reaction chambers and are manipulated using the electrowetting effect. Glucose is measured using a colorimetric enzyme-kinetic method based on Trinder's reaction. The color change is detected using an absorbance measurement system consisting of a light emitting diode and a photodiode. The linear range of the assay is 9-100 mg/dl using a sample dilution factor of 2 and 15-300 mg/dl using a sample dilution factor of 3. The results obtained on the electrowetting system compare favorably with conventional measurements done on a spectrophotometer, indicating that there is no change in enzyme activity under electrowetting conditions. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：145 / 150

页数：6