In vitro effect of adenosine A2A receptor antagonist SCH 442416 on the expression of glutamine synthetase and glutamate aspartate transporter in rat retinal Muller cells at elevated hydrostatic pressure

The aim of this study was to investigate the effect of an adenosine A(2A) receptor antagonist on the expression of glutamine synthetase (GS) and glutamate aspartate transporter (GLAST) in rat retinal Muller cells at elevated hydrostatic pressure in vitro. Immunofluorescence staining of GS and GFAP was used for the identification of Muller cells. The expression of GS and GLAST in different hydrostatic pressure (0, 20, 40, 60, 80 mmHg/24 h) was examined by real-time PCR and Western blotting to identify the most suitable pressure. Miller cells treated with 0.1, 1, 10 mu M SCH 442416 (A(2A) receptor antagonist) in the most suitable pressure, and the levels of GS and GLAST were examined by real-time PCR and Western blotting. Significantly increased expression of GS and GLAST at 40 mmHg pressure was observed in Muller cells and treatment with 10 mu M SCH 442416 in 40 mmHg pressure further promoted the expression of GS and GLAST. A receptor antagonist increased the expression of GLAST and GS of Muller cells and accelerated the clearance of extracellular glutamate.