Culture-independent analysis of fecal microbiota in cattle

被引：52
作者：
Ozutsumi, Y
Hayashi, H [1 ]
Sakamoto, M
Itabashi, H
Benno, Y
机构：
[1] Tokyo Univ Agr & Technol, United Grad Sch Agr Sci, Fuchu, Tokyo 1838509, Japan
[2] RIKEN, BioResource Ctr, Microbe Div Japan Collect Microorganisms, Wako, Saitama 3510198, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2005年 / 69卷 / 09期
关键词：
cattle; fecal bacteria; 16S rRNA; gene library; phylogenetic analysis;
D O I：
10.1271/bbb.69.1793
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The phylogenetic diversity of the fecal bacterial community in Holstein cattle was determined by 16S ribosomal RNA gene sequence analysis. The sequences were affiliated with the following phyla: Firmicutes (81.3%), Bacteroidetes (14.4%), Actinobacteria (2.5%), and Proteobacteria (1.4%). The Clostridium leptum subgroup was the most phylogenetically diverse group in cattle feces. In addition, a number of previously uncharacterized and unidentified bacteria were recognized in clone libraries.
引用
收藏
页码：1793 / 1797
页数：5
相关论文
共 13 条
  • [1] BASIC LOCAL ALIGNMENT SEARCH TOOL
    ALTSCHUL, SF
    GISH, W
    MILLER, W
    MYERS, EW
    LIPMAN, DJ
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) : 403 - 410
  • [2] [Anonymous], [No title captured], DOI [10.12938/BIFIDUS1982.2.1_41, DOI 10.12938/BIFIDUS1982.2.1_41]
  • [3] Identification of nonpoint sources of fecal pollution in coastal waters by using host-specific 16S ribosomal DNA genetic markers from fecal anaerobes
    Bernhard, AE
    Field, KG
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (04) : 1587 - 1594
  • [4] Isolating PCR-quality DNA from human feces with a soil DNA kit
    Clement, BG
    Kitts, CL
    [J]. BIOTECHNIQUES, 2000, 28 (04) : 640 - +
  • [5] The Ribosomal Database Project (RDP-II): previewing a new autoaligner that allows regular updates and the new prokaryotic taxonomy
    Cole, JR
    Chai, B
    Marsh, TL
    Farris, RJ
    Wang, Q
    Kulam, SA
    Chandra, S
    McGarrell, DM
    Schmidt, TM
    Garrity, GM
    Tiedje, JM
    [J]. NUCLEIC ACIDS RESEARCH, 2003, 31 (01) : 442 - 443
  • [6] Lane D.J., 1991, NUCL ACID TECHNIQUES, P177
  • [7] Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited
    Leser, TD
    Amenuvor, JZ
    Jensen, TK
    Lindecrona, RH
    Boye, M
    Moller, K
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (02) : 673 - 690
  • [8] O'Sullivan DJ, 1999, PROBIOTICS: A CRITICAL REVIEW, P23
  • [9] THE NEIGHBOR-JOINING METHOD - A NEW METHOD FOR RECONSTRUCTING PHYLOGENETIC TREES
    SAITOU, N
    NEI, M
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 1987, 4 (04) : 406 - 425
  • [10] A PLACE FOR DNA-DNA REASSOCIATION AND 16S RIBOSOMAL-RNA SEQUENCE-ANALYSIS IN THE PRESENT SPECIES DEFINITION IN BACTERIOLOGY
    STACKEBRANDT, E
    GOEBEL, BM
    [J]. INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1994, 44 (04): : 846 - 849
12