Association of single nucleotide polymorphisms in the CAPN, CAST, LEP, GH, and IGF-1 genes with growth parameters and ultrasound characteristics of the Longissimus dorsi muscle in Colombian hair sheep

Recognition of the genes that influence livestock production characteristics has allowed researchers to identify single nucleotide polymorphisms (SNPs) associated with phenotypic traits that contribute to higher productivity. The objective of this research was to associate SNPs in the genes calpain (CAPN), calpastatin (CAST), leptin (LEP), growth hormone (GH), and insulin-like growth factor 1 (IGF-1) with the growth characteristics birth weight (BW), weaning weight adjusted at 120 days (WW), daily pre-weaning gain (PRADG), adjusted weight at 210 days (AW210), and daily post-weaning gain (POADG), and the measures of the Longissimus dorsi muscle based on ultrasound, namely loin eye area (LEA), loin depth (LD), and back fat thickness (BFT), in Colombian hair sheep (OPC). The association between phenotypic and genotypic characteristics was made using the PLINK v.1.9 program using linear regression analysis. There was a statistically significant association (p < 0.05) between the CAST polymorphism (M/N) and BW, a tendency (p = 0.07) for an association between the T -> C SNP of the CAPN gene and AW210, and a trend (p = 0.07) for an association between the A -> G SNP of the IGF-1 locus and POADG. The LEA and BFT characteristics were not associated with a SNP, while PL was significantly affected by SNPs in the GH and IGF-1 genes. In conclusion, all the genes evaluated were polymorphic, the CAST gene significantly influenced BW, and the GH and IGF-1 genes were associated with LD characteristics. These results could be used to identify individuals with favorable genotypes to implement a marker-assisted selection method.