共 52 条
Stress-responsive maturation of Clk1/4 pre-mRNAs promotes phosphorylation of SR splicing factor
被引:123
作者:
Ninomiya, Kensuke
[1
]
Kataoka, Naoyuki
[1
]
Hagiwara, Masatoshi
[1
]
机构:
[1] Kyoto Univ, Grad Sch Med, Dept Anat & Dev Biol, Sakyo Ku, Kyoto 6068501, Japan
基金:
日本科学技术振兴机构;
关键词:
HEAT-SHOCK PROTEINS;
INTRANUCLEAR DISTRIBUTION;
CELL-CYCLE;
RS DOMAIN;
IN-VIVO;
KINASE;
SERINE;
CLONING;
FAMILY;
RICH;
D O I:
10.1083/jcb.201107093
中图分类号:
Q2 [细胞生物学];
学科分类号:
071009 ;
090102 ;
摘要:
It has been assumed that premessenger ribonucleic acids (RNAs; pre-mRNAs) are spliced cotranscriptionally in the process of gene expression. However, in this paper, we report that splicing of Clk1/4 mRNAs is suspended in tissues and cultured cells and that intermediate forms retaining specific introns are abundantly pooled in the nucleus. Administration of the Cdc2-like kinase-specific inhibitor TG003 increased the level of Clk1/4 mature mRNAs by promoting splicing of the intron-retaining RNAs. Under stress conditions, splicing of general pre-mRNAs was inhibited by dephosphorylation of SR splicing factors, but exposure to stresses, such as heat shock and osmotic stress, promoted the maturation of Clk1/4 mRNAs. Clk1/4 proteins translated after heat shock catalyzed rephosphorylation of SR proteins, especially SRSF4 and SRSF10. These findings suggest that Clk1/4 expression induced by stress-responsive splicing serves to maintain the phosphorylation state of SR proteins.
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页码:27 / 40
页数:14
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