Determination of hydroxyl radicals in Fenton reaction by colorimetric assay and its application.

被引：0

作者：

Xu, XR ^{[1
]}

Wang, WH ^{[1
]}

Li, HB ^{[1
]}

机构：

[1] Chinese Acad Sci, Ecoenvironm Sci Res Ctr, State Key Lab Environm Aquat Chem, Beijing 100085, Peoples R China

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 1999年 / 26卷 / 01期

关键词：

hydroxyl radical; Fenton reaction; colorimetry; methane sulfinic acid;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hydroxyl radicals produced in Fenton reaction can react with dimethyl sulfoxide to produce methane sulfinic acid which can further react with Fast Blue BE salt to form light yellow diazosulfone. According to the principle, hydroxyl radicals can be indirectly assayed by colorimetry. The determination condition was studied to obtain a best experimental program. The results showed that the hydroxyl-radical-scavenging effect were dose dependent upon thiourea and ascorbic acid. The walnut and black sesame were proved to be natural anti-oxidation foods. The method can be applied to sieve the hydroxyl radical scavenger.

引用

页码：67 / 69

页数：3

共 6 条

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