Functional characteristics of pyruvate transport in Phycomyces blakesleeanus

A saturable and accumulative transport system for pyruvate has been detected in Phycomyces blakesleeanus NRRL 1555(-) mycelium, It was strongly inhibited by alpha-cyano-4-hydroxycinnamate, L-Lactate and acetate were competitive inhibitors of pyruvate transport. The initial pyruvate uptake velocity and accumulation ratio was dependent on the external pH, The V-max of transport greatly decreased with increasing pH, whereas the affinity of the carrier for pyruvate was not affected, The pyruvate transport system mediated its homologous exchange, which was essentially pH independent, and efflux, which increased with increasing external pH, The uptake of pyruvate was energy dependent and was strongly inhibited by inhibitors of oxidative phosphorylation and of the formation of proton gradients. Glucose counteracted the inhibitory effect of the pyruvate transport produced by inhibitors of mitochondrial ATP synthesis. Our results are consistent with a pyruvate/proton cotransport in P, blakesleeanus probably driven by an electrochemical gradient of H+ generated by a plasma membrane H+-ATPase, (C) 1998 Academic Press.