Sperm DNA fragmentation in the total and vital fractions before and after density gradient centrifugation: Significance in male fertility diagnosis

被引：15
作者：
Punjabi, U. [1 ]
Van Mulders, H. [1 ]
Goovaerts, I [1 ]
Peeters, K. [1 ]
Clasen, K. [1 ]
Janssens, P. [1 ]
Zemtsova, O. [1 ]
De Neubourg, D. [1 ]
机构：
[1] Antwerp Univ Hosp, Ctr Reprod Med, Wilrijkstr 10, B-2650 Edegem, Belgium
来源：
CLINICAL BIOCHEMISTRY | 2018年 / 62卷
关键词：
Total sperm DNA fragmentation; Vital sperm DNA fragmentation; TUNEL assay; Density gradient centrifugation; DEOXYRIBONUCLEIC-ACID FRAGMENTATION; CHROMATIN DISPERSION-TEST; IN-VITRO FERTILIZATION; SEMEN ANALYSIS COURSES; OXIDATIVE STRESS; HUMAN SPERMATOZOA; MALE-INFERTILITY; INTRAUTERINE INSEMINATION; BLASTOCYST DEVELOPMENT; DAMAGE;
D O I：
10.1016/j.clinbiochem.2018.05.011
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: Sperm DNA fragmentation measured by different techniques make comparisons impossible due to lack of standardization. Induction of DNA damage after sperm preparation in the entire fraction has been observed on independent occasions but findings are not consistent. Methods: Men presenting at a University hospital setup for infertility treatment. DNA damage via TUNEL assay was validated on fresh semen samples, as conventional semen parameters, to reduce variability of results. Results: Sperm motility in neat semen inversely correlated with sperm DNA fragmentation in the total fraction, but, total count, leukocytes and immature germ cells significantly affected the vital fraction. Sperm DNA fragmentation was observed both in normal and subnormal semen samples, but was significantly different in the total fraction of astheno-, asthenoterato- and oligoteratozoospermic men. After density gradient centrifugation, sperm DNA fragmentation increased significantly in the total but decreased in the vital fraction. Advancing male age significantly influenced damage in the total but not in the vital population. Conclusions: These findings provide opportunities to investigate the significance of the total and the vital fractions both in natural conception and after different assisted reproductive technologies.
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页码：47 / 54
页数：8
相关论文
共 88 条
[1]   Oxidative stress, DNA damage and apoptosis in male infertility: a clinical approach [J].
Agarwal, A ;
Said, TM .
BJU INTERNATIONAL, 2005, 95 (04) :503-507
[2]   Potential importance of transition metals in the induction of DNA damage by sperm preparation media [J].
Aitken, R. J. ;
Finnie, J. M. ;
Muscio, L. ;
Whiting, S. ;
Connaughton, H. S. ;
Kuczera, L. ;
Rothkirch, T. B. ;
De Iuliis, G. N. .
HUMAN REPRODUCTION, 2014, 29 (10) :2136-2147
[3]  
Aitken R. J., 1995, HUM REPROD, V10, P261
[4]   Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development [J].
Aitken, R. John ;
Baker, Mark A. .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY, 2013, 57 (2-4) :265-272
[5]   Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: development of diagnostic criteria [J].
Aitken, R. John ;
De Iuliis, Geoffry N. ;
Finnie, Jane M. ;
Hedges, Andrew ;
McLachlan, Robert I. .
HUMAN REPRODUCTION, 2010, 25 (10) :2415-2426
[6]   Biological and clinical significance of DNA damage in the male germ line [J].
Aitken, R. John ;
De Iuliis, Geoffry N. ;
McLachlan, Robert I. .
INTERNATIONAL JOURNAL OF ANDROLOGY, 2009, 32 (01) :46-56
[7]  
Aitken RJ, 1999, J REPROD FERTIL, V115, P1
[8]   SEMINAL LEUKOCYTES - PASSENGERS, TERRORISTS OR GOOD-SAMARITANS [J].
AITKEN, RJ ;
BAKER, HWG .
HUMAN REPRODUCTION, 1995, 10 (07) :1736-1739
[9]   ANALYSIS OF THE RELATIONSHIP BETWEEN REACTIVE OXYGEN SPECIES PRODUCTION AND LEUKOCYTE INFILTRATION IN FRACTIONS OF HUMAN SEMEN SEPARATED ON PERCOLL GRADIENTS [J].
AITKEN, RJ ;
WEST, KM .
INTERNATIONAL JOURNAL OF ANDROLOGY, 1990, 13 (06) :433-451
[10]   Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa [J].
Aitken, RJ ;
Gordon, E ;
Harkiss, D ;
Twigg, JP ;
Milne, P ;
Jennings, Z ;
Irvine, DS .
BIOLOGY OF REPRODUCTION, 1998, 59 (05) :1037-1046
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