In Vitro Antifungal Activity of Peltophorum dubium (Spreng.) Taub. extracts against Aspergillus flavus

被引：15
作者：
Di Ciaccio, Lucia S. [1 ]
Catalano, Alejandra V. [2 ,3 ]
Lopez, Paula G. [2 ,3 ]
Rojas, Dante [4 ]
Cristos, Diego [4 ]
Fortunato, Renee H. [2 ,5 ,6 ]
Salvat, Adriana E. [1 ]
机构：
[1] Inst Nacl Tecnol Agr, CICVyA, Inst Patobiol Vet IPvet, RA-1686 Hurlingham, Buenos Aires, Argentina
[2] Consejo Nacl Invest Cient & Tecn CONICET, RA-1425 Buenos Aires, DF, Argentina
[3] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Farmacognosia, RA-1113 Buenos Aires, DF, Argentina
[4] Inst Nacl Tecnol Agr, Inst Tecnol Alimentos, RA-1686 Hurlingham, Buenos Aires, Argentina
[5] Inst Nacl Tecnol Agr, CIRN, Inst Recursos Biol, RA-1686 Hurlingham, Buenos Aires, Argentina
[6] Univ Moron, Fac Agron & Ciencias Agroalimentarias, RA-1708 Moron, Buenos Aires, Argentina
来源：
PLANTS-BASEL | 2020年 / 9卷 / 04期
关键词：
Aspergillus flavus; flavonoids; plant bioactivity; evans blue; active extract characterization; THYME ESSENTIAL OILS; FUSARIUM-VERTICILLIOIDES; AFLATOXIN PRODUCTION; OREGANO; GROWTH; L;
D O I：
10.3390/plants9040438
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Aspergillus flavus is a filamentous, saprophytic fungus, whose colonization occurs mainly in cereal grains and oilseeds once harvested. Under certain conditions, it could produce mycotoxins called aflatoxins, known as powerful human liver carcinogens. The aim of the present study was to describe the antifungal activity of extracts of Peltophorum dubium, a species from northern Argentina (Oriental Chaco), against A. flavus. The antifungal activities of different collection sites are reported. The extracts exhibited a minimum inhibitory concentration of 125 mu g/mL, and the differences between the treatments and the inoculum control were 11 mm of P. dubium A and 10 mm of P. dubium F in colony growth. Moreover, hyphae treated with the extracts stained blue with Evans blue showed alterations in the membrane and/or cell wall, allowing the dye income. Bio-guided fractionation, High Performance Liquid Chromatography diode array ultraviolet/visible (HPLC UV/VIS DAD), and Ultra-High Performance Liquid Chromatography Electrospray Ionization Mass Spectrometry (UPLC ESI-MS) analyses were conducted to characterize the extracts and their active fractions. The HPLC UV/VIS DAD analysis allowed the determination of the presence of flavonoids (flavonols and flavones), coumarins, terpenes, and steroids. UPLC ESI/MS analysis of active fractions revealed the presence of Kaempferol, Apigenin, Naringenin, Chrysin and Daidzein.
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页数：11
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