Role of formate hydrogen lyase complex in hydrogen production in facultative anaerobes

被引:36
作者
Sinha, Pallavi [1 ]
Roy, Shantonu [1 ]
Das, Debabrata [1 ]
机构
[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India
关键词
Facultative anaerobes; Formate hydrogen lyase; Formate dehydrogenase; Transporter proteins; Hydrogenase; ESCHERICHIA-COLI; BIOHYDROGEN PRODUCTION; TRANSCRIPTIONAL ACTIVATOR; H-2; PRODUCTION; CLOSTRIDIUM-BUTYRICUM; KLEBSIELLA-PNEUMONIAE; METHANE PRODUCTION; OPERON; PH; DEHYDROGENASE;
D O I
10.1016/j.ijhydene.2015.05.076
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Facultative anaerobes play important roles in H-2 production by biological routes. In the present study, a comprehensive insight on the importance of formate lyase in H-2 production by facultative microorganisms has been focused. Structural details of formate hydrogen lyase reveal the involvement of two enzymes, formate dehydrogenase H (FdhH) and hydrogenase (Hyd) along with electron carriers. The monocistronic fdhF gene codes for the functional formate dehydrogenase H. Functionality of these complexes are dependent on maturation system governed by FDH-D and FDH-E. The overall transcription of FHL complex is governed by protein encoded by fhlA gene. Under anaerobic condition, the pyruvate gets cleave into acetyl-CoA and formate by pyruvate-formate-lyase (PFL). The transporter proteins FocA and FocB helps in transporting formate. During dark fermentation, the physico-chemical parameters such as pH, HRTs, partial pressure play a crucial role in influencing formate degradation to molecular H-2. Copyright (C) 2015, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:8806 / 8815
页数:10
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