Effect of manoalide on apoptosis induced by deprivation of growth factors in vascular endothelial cells

被引：0

作者：

Miao, JY ^{[1
]}

Araki, S

Zhang, HW

Hayashi, H

机构：

[1] Shandong Univ, Sch Life Sci, Inst Dev Biol, Jinan 250100, Peoples R China

[2] Nagoya Univ, Sch Sci, Sugashima Marine Biol Lab, Toba, Mie 517, Japan

来源：

ACTA PHARMACOLOGICA SINICA | 1999年 / 20卷 / 02期

关键词：

manoalide; apoptosis; umbilical veins; vascular endothelium; cultured cells; cell survival; DNA fragmentation; acidic fibroblast growth factor;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

AIM: To study effect of manoalide on apoptosis induced by deprivation of acidic fibroblast growth factor (aFGF) and serum in vascular endothelial cells ( VEC). METHODS: Morphologic changes were observed by light microscopy. Viability was determined by counting the cells that attached to dishes after treatments. DNA fragmentation was analyzed by agarose gel electrophoresis and fluorescence microscopy. RESULTS: The cells deprived of aFGF and serum were exposed to manoalide 1 - 4 mu mol.L-1 for 48 h, detachment and DNA fragmentation of these cells were suppressed. At 7 mu mol.L-1, manoalide promoted detachment and DNA fragmentation of VEC. CONCLUSION: manoalide 2 mu mol L-1 inhibited, but 7 mu mol.L-1 promoted, apoptosis of VEC.

引用

页码：121 / 125

页数：5

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