Evaluation of DNA Damage Using 3 Comet Assay Parameters in Workers Occupationally Exposed to Lead

被引：20
作者：
Kayaalti, Zeliha [1 ]
Yavuz, Ilknur [1 ]
Soylemez, Esma [1 ]
Bacaksiz, Aysegul [2 ]
Tutkun, Engin [3 ]
Sayal, Ahmet [4 ]
Soylemezoglu, Tulin [1 ]
机构：
[1] Ankara Univ, Inst Forens Sci, TR-06590 Ankara, Turkey
[2] Kastamonu Univ, Fazil Boyner Sch Hlth, Dept Nutr & Dietet, Kastamonu, Turkey
[3] Ankara Occupat Dis Hosp, Ankara, Turkey
[4] Gulhane Mil Med Acad, Dept Toxicol, Ankara, Turkey
来源：
ARCHIVES OF ENVIRONMENTAL & OCCUPATIONAL HEALTH | 2015年 / 70卷 / 03期
关键词：
occupational exposure; lead; cigarette smoking; DNA damage; comet assay; INDUCED GENOTOXICITY; STRAND BREAKS; LYMPHOCYTES; ABERRATIONS; INHIBITION; CADMIUM; CANCER; COPPER; CELLS;
D O I：
10.1080/19338244.2013.787964
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
The aim of this study was to investigate the association between DNA damage and blood lead levels in individuals occupationally exposed to lead. To evaluate this association, 61 workers exposed to lead were monitored in terms of DNA damage in blood lymphocytes. The levels of DNA damage were measured according to 3 comet assay parameters, including tail intensity (TI), tail moment (TM), and DNA tail (DNAt). A statistically significant positive correlation was found between the lead levels and TI, TM, and DNAt (p <.01). Smoking had independent effects on DNA damage. A statistically significant difference was observed between smokers and nonsmokers in regards to DNA damage parameters (p <.05). In addition, the lead and DNA damage levels in smokers were found to be significantly higher than the levels observed in nonsmoking workers (p <.05). Our results show that exposure to lead induces genotoxic effects in peripheral lymphocytes, as measured by comet assays.
引用
收藏
页码：120 / 125
页数：6
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