Arabidopsis Mitochondrial Voltage-Dependent Anion Channels Are Involved in Maintaining Reactive Oxygen Species Homeostasis, Oxidative and Salt Stress Tolerance in Yeast

被引：25

作者：

Sanyal, Sibaji K. ^{[1
]}

Kanwar, Poonam ^{[1
]}

Fernandes, Joel Lars ^{[1
]}

Mahiwal, Swati ^{[1
]}

Yadav, Akhilesh K. ^{[1
,3
]}

Samtani, Harsha ^{[1
]}

Srivastava, Ashish K. ^{[2
]}

Suprasanna, Penna ^{[2
]}

Pandey, Girdhar K. ^{[1
]}

机构：

[1] Univ Delhi, Dept Plant Mol Biol, South Campus, New Delhi, India

[2] Bhabha Atom Res Ctr, Nucl Agr & Biotechnol Div, Mumbai, Maharashtra, India

[3] Shri Murli Manohar Town PG Coll, Ballia, India

来源：

FRONTIERS IN PLANT SCIENCE | 2020年 / 11卷

关键词：

Por1; mitochondria; reactive oxygen species; stress; mitochondrial membrane potential; Arabidopsis; HUMAN VDAC ISOFORMS; PROTEIN-KINASE; GENE FAMILY; MEMBRANE; EXPRESSION; CELL; INTERACTS; PORIN; TRANSLATION; RESPIRATION;

D O I：

10.3389/fpls.2020.00050

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Voltage-dependent anion channels (VDACs) are conserved proteins of the mitochondria. We have functionally compared Arabidopsis VDACs using Saccharomyces cerevisiae Delta por1 and M3 yeast system. VDAC (1, 2, and 4) were able to restore Delta por1 growth in elevated temperature, in oxidative and salt stresses, whereas VDAC3 only partially rescued Delta por1 in these conditions. The ectopic expression of VDAC (1, 2, 3, and 4) in mutant yeast recapitulated the mitochondrial membrane potential thus, enabled it to maintain reactive oxygen species homeostasis. Overexpression of these VDACs (AtVDACs) in M3 strain did not display any synergistic or antagonistic activity with the native yeast VDAC1 (ScVDAC1). Collectively, our data suggest that Arabidopsis VDACs are involved in regulating respiration, reactive oxygen species homeostasis, and stress tolerance in yeast.

引用

页数：14

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