Estimation of DNA single-strand breaks by single cells gel electrophoresis in tumor cells

被引：1

作者：

Neubauer, S

Liehr, T

Birkenhake, S

Gebhart, E

Fietkau, R

Sauer, R

机构：

[1] Radiotherapeuth Clin & Policlin, D-91054 Erlangen, Germany

[2] Inst Human Genet, D-07743 Jena, Germany

[3] Inst Human Genet, D-91054 Erlangen, Germany

[4] Radiotherapeuth Clin & Policlin, D-18958 Rostock, Germany

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1998年 / 14卷 / 04期

关键词：

comet assay; DNA-damage; DNA-repair; tumor cells;

D O I：

10.1016/S1050-3862(98)00009-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To estimate the frequency of single-strand breaks on a single cell level the so-called comet-assay (single cell gel electrophoresis) is a well-established technique. We present a modified protocol suitable for testing primary tumors, a kind of tissue very uneasy to be analysed in former single cell gel electrophoresis assays. Tumor cells of 12 studied cases showed a typical dose-rate effect on in vitro irradiatiation with different X-ray doses, as observed in peripheral blood leukocytes. Interestingly, the repair capability of primary tumor cells was lower than that of peripheral blood leukocytes of the same patients. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：121 / 124

页数：4

共 11 条

[1] Use of the alkaline comet assay to detect DNA repair deficiencies in human fibroblasts exposed to UVC, UVB, UVA and gamma-rays [J].