Redox-Activated Contrast-Enhanced T1-Weighted Imaging Visualizes Glutathione-Mediated Biotransformation Dynamics in the Liver

被引：17
作者：
Liu, Kun [1 ,2 ]
Kang, Bilun [1 ,2 ]
Luo, Xiangjie [1 ,2 ]
Yang, Zhaoxuan [1 ,2 ]
Sun, Chengjie [1 ,2 ]
Li, Ao [1 ,2 ]
Fan, Yifan [1 ,2 ]
Chen, Xiaoyuan [3 ,4 ]
Gao, Jinhao [1 ,2 ]
Lin, Hongyu [1 ,2 ]
机构：
[1] Xiamen Univ, MOE Key Lab Spectrochem Anal & Instrumentat, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn,Key Lab Chem Biol Fujian Pr, Xiamen 361005, Peoples R China
[2] Xiamen Univ, Coll Chem & Chem Engn, Dept Chem Biol, Xiamen 361005, Peoples R China
[3] Natl Univ Singapore, Yong Loo Lin Sch Med, Singapore 119228, Singapore
[4] Natl Univ Singapore, Fac Engn, Singapore 119228, Singapore
来源：
ACS NANO | 2021年 / 15卷 / 11期
基金：
中国国家自然科学基金;
关键词：
redox-activated probes; glutathione; biotransformations; magnetic resonance imaging; liver functions; NANOPARTICLES; TRANSPORT; SURFACE; EFFLUX; PROBES;
D O I：
10.1021/acsnano.1c06026
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
GSH-mediated liver biotransformation is a crucial physiological process demanding efficient research tools. Here, we report a type of amorphous FexMnyO nanoparticles (AFMO-ZDS NPs) as redox-activated probes for in vivo visualization of the dynamics of GSH-mediated biotransformation in liver with T-1-weighted magnetic resonance imaging (MRI). This imaging technique reveals the periodic variations in GSH concentration during the degradation of AFMO-ZDS NPs due to the limited transportation capacity of GSH carriers in the course of GSH efflux from hepatocytes to perisinusoidal space, providing direct imaging evidence for this important carrier-mediated process during GSH-mediated biotransformation. Therefore, this technique offers an effective method for in-depth investigations of GSH-related biological processes in liver under various conditions as well as a feasible means for the real-time assessment of liver functions, which is highly desirable for early diagnosis of liver diseases and prompt a toxicity evaluation of pharmaceuticals.
引用
收藏
页码：17831 / 17841
页数：11
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