A first glycoside hydrolase family 50 endo-β-1,3-D-glucanase from Pseudomonas aeruginosa

被引：16

作者：

Yi, Ping ^{[1
]}

Yan, Qiaojuan ^{[1
]}

Jiang, Zhengqiang ^{[2
]}

Wang, Ling ^{[2
]}

机构：

[1] China Agr Univ, Coll Engn, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing 100083, Peoples R China

[2] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2018年 / 108卷

基金：

中国国家自然科学基金;

关键词：

Endo-1,3-beta-D-glucanase; Curdlan; Pseudomonas aeruginosa; Glycoside hydrolase family 50; ESCHERICHIA-COLI; BIOCHEMICAL-CHARACTERIZATION; FUNCTIONAL-CHARACTERIZATION; ANTIFUNGAL ACTIVITY; MOLECULAR-CLONING; PAENIBACILLUS SP; BETA-AGARASE; BETA-1,3-GLUCANASE; CURDLAN; ENDO-BETA-1,3-GLUCANASE;

D O I：

10.1016/j.enzmictec.2017.09.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel beta-1,3-glucanase gene (PaBglu50A) from Pseudomonas aeruginosa CAU 342A was cloned and expressed in Escherichia coil. The deduced amino acid sequence of PaBglu50A showed the highest identity of 34% with the beta-agarase belonging to glycoside hydrolase (GH) family 50. The purified PaBglu50A had maximal activity at pH 5.5 and 45 degrees C, respectively. It was stable in the range of pH 4.0-8.0 and at temperatures below 40 degrees C. The K-m and V-max of PaBglu50A for curdlan and laminarin were 94.4 mg ml(-1) and 23.4 mu mol min(-1) mg(-1), 3.65 mg ml(-1) and 8.89 mu mol min(-1) mg(-1), respectively. All characterized members of GH family 50 were only active towards agarose so far. However, the recombinant protein PaBglu50A did not display activity towards agarose but showed activity towards water-insoluble curdlan and laminarin. The hydrolysis products for curdlan supported this protein to be an endo-beta-1,3-glucanase, making a significant difference from the reported enzymes of GH family 50. These results suggested that PaBglu50A is the first endo-type beta-1,3-glucanase (EC 3.2.1.39) in GH family 50.

引用

页码：34 / 41

页数：8