Industrial yeast strain engineered to ferment ethanol from lignocellulosic biomass

被引：38

作者：

Khramtsov, Nikolai ^{[1
]}

McDade, Luise ^{[1
]}

Amerik, Alexander ^{[1
]}

Yu, Esther ^{[1
]}

Divatia, Kunjan ^{[1
]}

Tikhonov, Alexander ^{[1
]}

Minto, Michael ^{[1
]}

Kabongo-Mubalamate, Georges ^{[1
]}

Markovic, Zdenek ^{[1
]}

Ruiz-Martinez, Marie ^{[1
]}

Henck, Steven ^{[1
]}

机构：

[1] Arbor Fuel Inc, Woodbridge, CT 06525 USA

来源：

BIORESOURCE TECHNOLOGY | 2011年 / 102卷 / 17期

关键词：

Cellulolytic industrial yeast; Corn stover; Cellulases; Simultaneous saccharification and fermentation (SSF); Ethanol production; SACCHAROMYCES-CEREVISIAE; CELLULOSE; HYDROLYSIS; CONVERSION;

D O I：

10.1016/j.biortech.2011.05.075

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

In this study an industrial Saccharomyces cerevisiae yeast strain capable of fermenting ethanol from pretreated lignocellulosic material was engineered. Genes encoding cellulases (endoglucanase, exoglucanase and beta-glucosidase) were integrated into the chromosomal ribosomal DNA and delta regions of a derivative of the K1-V1116 wine yeast strain. The engineered cellulolytic yeast produces ethanol in one step through simultaneous saccharification and fermentation of pretreated biomass without the addition of exogenously produced enzymes. When ethanol fermentation was performed with 10% dry weight of pretreated corn stover, the recombinant strain fermented 63% of the cellulose in 96 h and the ethanol titer reached 2.6% v/v. These results demonstrate that cellulolytic S. cerevisiae strains can be used as a platform for developing an economical advanced biofuel process. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：8310 / 8313

页数：4

共 12 条

[1] Hydrolysis and fermentation of amorphous cellulose by recombinant Saccharomyces cerevisiae [J].