Resistance to Puccinia graminis f. sp avenae in Barley Is Associated with the Rpg5 Locus

被引：7
作者：
Dracatos, Peter [1 ]
Singh, Davinder [1 ]
Fetch, Tom, Jr. [2 ]
Park, Robert [1 ]
机构：
[1] Univ Sydney, Plant Breeding Inst Cobbitty, Narellan, NSW 2567, Australia
[2] Agr & Agri Food Canada, Cereal Res Ctr, Winnipeg, MB, Canada
来源：
PHYTOPATHOLOGY | 2015年 / 105卷 / 04期
基金：
澳大利亚研究理事会;
关键词：
STEM RUST-RESISTANCE; SP TRITICI; GENE RPG1; WHEAT; INHERITANCE; HYBRIDIZATION; TEMPERATURE; SECALIS;
D O I：
10.1094/PHYTO-08-14-0224-R
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
In barley, gene Rpg5 was first identified for providing resistance to the rye stem rust pathogen (Puccinia graminis f. sp. secalis). A subsequent study determined that Rpg5 is required for rpg4-mediated resistance to the wheat stem rust pathogen (P. graminis f. sp. tritici) including pathotype TTKSK ("Ug99"), which poses a major threat to global wheat and barley production. Based on the effectiveness of Rpg5 against P. graminis f. sp. tritici and P. graminis f. sp. secalis, we assessed whether it also conferred resistance to the oat stem rust pathogen (P. graminis f. sp. avenae). A barley F8 recombinant inbred line (RIL) population was produced by crossing 'Q21861' (Rpgl and Rpg5) with '73-G1' (Rpgl), which is susceptible to P. graminis f. sp. avenae, P. graminis f. sp. secalis, and some pathotypes of P. graminis f. sp. tritici. Seedling tests were performed on the F8 RIL population using Australian pathotypes of P. graminis f. sp. tritici, P. graminis f. sp. secalis, P. graminis f. sp. avenae, and a putative somatic hybrid between P. graminis f. sp. tritici and P. graminis f. sp. secalis known as the 'Scabrum' rust. Segregation in the responses to all rust isolates for the RILs was identical (50 resistant: 52 susceptible), and fitted a 1: 1 ratio (X-2 = 0.039, P = 0.843), indicating that resistance to all isolates was monogenetically inherited. Screening of the RILs and the parental lines with perfect markers for the functional Rpgl and Rpg5 resistance alleles indicated that Rpgl was fixed, while Rpg5 was positive in all resistant lines and negative in all susceptible lines. This suggests that different formae speciales of P. graminis may share common effectors, and that the Rpg5 locus confers resistance to both P. graminis f. sp. tritici and P. graminis f. sp. secalis and the heterologous formae speciales of P. graminis, P. graminis f. sp. avenae.
引用
收藏
页码：490 / 494
页数：5
相关论文
共 34 条
[1]  
Anikster Y., 1984, The cereal rusts, P115, DOI [10.1016/B978-0-12-148401-9.50010-8, DOI 10.1016/B978-0-12-148401-9.50010-8]
[2]   Allele Characterization of Genes Required for rpg4-Mediated Wheat Stem Rust Resistance Identifies Rpg5 as the R Gene [J].
Arora, D. ;
Gross, T. ;
Brueggeman, R. .
PHYTOPATHOLOGY, 2013, 103 (11) :1153-1161
[3]   Accumulation of genes for susceptibility to rust fungi for which barley is nearly a nonhost results in two barley lines with extreme multiple susceptibility [J].
Atienza, SG ;
Jafary, H ;
Niks, RE .
PLANTA, 2004, 220 (01) :71-79
[4]   The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains [J].
Brueggeman, R. ;
Druka, A. ;
Nirmala, J. ;
Cavileer, T. ;
Drader, T. ;
Rostoks, N. ;
Mirlohi, A. ;
Bennypaul, H. ;
Gill, U. ;
Kudrna, D. ;
Whitelaw, C. ;
Kilian, A. ;
Han, F. ;
Sun, Y. ;
Gill, K. ;
Steffenson, B. ;
Kleinhofs, A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (39) :14970-14975
[5]   The barley stem rust-resistance gene Rpg1 is a novel disease-resistance gene with homology to receptor kinases [J].
Brueggeman, R ;
Rostoks, N ;
Kudrna, D ;
Kilian, A ;
Han, F ;
Chen, J ;
Druka, A ;
Steffenson, B ;
Kleinhofs, A .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (14) :9328-9333
[6]   The rpg4/Rpg5 stem rust resistance locus in barley Resistance genes and cytoskeleton dynamics [J].
Brueggeman, Robert ;
Steffenson, Brian J. ;
Kleinhofs, Andris .
CELL CYCLE, 2009, 8 (07) :977-981
[7]   Analysis of Stem Rust Resistance in Australian Barley Cultivars [J].
Derevnina, L. ;
Fetch, T. ;
Singh, D. ;
Brueggeman, R. ;
Dong, C. ;
Park, R. F. .
PLANT DISEASE, 2014, 98 (11) :1485-1493
[8]   STEM RUST EPIDEMICS AND THEIR EFFECTS ON GRAIN-YIELD AND QUALITY IN AUSTRALIAN BARLEY CULTIVARS [J].
DILLMACKY, R ;
REES, RG ;
PLATZ, GJ .
AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH, 1990, 41 (06) :1057-1063
[9]   Inheritance of Prehaustorial Resistance to Puccinia graminis f. sp avenae in Barley (Hordeum vulgare L.) [J].
Dracatos, Peter M. ;
Ayliffe, Michael ;
Khatkar, Mehar S. ;
Fetch, Tom, Jr. ;
Singh, Davinder ;
Park, Robert F. .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2014, 27 (11) :1253-1262
[10]   Chromosomal Location and Inheritance of Stem Rust Resistance Transferred from Hordeum bulbosum into Cultivated Barley (H-vulgare) [J].
Fetch, T., Jr. ;
Johnston, P. A. ;
Pickering, R. .
PHYTOPATHOLOGY, 2009, 99 (04) :339-343
1234