Genome analysis of Thinopyrum intermedium and Thinopyrum ponticum using genomic in situ hybridization

被引：179

作者：

Chen, Q ^{[1
]}

Conner, RL ^{[1
]}

Laroche, A ^{[1
]}

Thomas, JB ^{[1
]}

机构：

[1] Agr Canada, Lethbridge Res Ctr, Lethbridge, AB T1J 4B1, Canada

来源：

GENOME | 1998年 / 41卷 / 04期

关键词：

genomic in situ hybridization; GISH; Thinopyrum intermedium; Thinopyrum ponticum; genomic analysis; J(s) genome;

D O I：

10.1139/gen-41-4-580

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Genomic in situ hybridization (GISH) using genomic DNA probes from Thinopyrum elongatum (Host) D.R. Dewey (genome E, 2n = 14), Thinopyrum bessarabicum (Savul. & Rayss) A. Love (genome J, 2n = 14), and Pseudoroegneria strigosa (M. Bieb.) A. Love (genome S, 2n = 14), was used to examine the genomic constitution of Thinopyrum intermedium (Host) Barkworth Sr D.R. Dewey (2n = 6x = 42) and Thinopyrum ponticllm (Podp.) Barkworth Sr D.R. Dewey (2n = 10x = 70). Evidence from GISH indicated that hexaploid Tit, intermedium contained the J, J(s), and S genomes, in which the J genome was related to the E genome of Th. elongatum and the J genome of Th. bessarabicum. The S genome was homologous to the S genome of Ps. strigosa, while the J(s) genome referred to modified J- or E-type chromosomes distinguished by the presence of S genome specific sequences close to the centromere. Decaploid Th. ponticum had only the two basic genomes J and J(s). The J(s) genome present in Th. intermedium and Th. ponticum was homologous with E or J genomes, but was quite distinct at centromeric regions, which can strongly hybridize with the S genome DNA probe. Based on GISH results, the genomic formula of Th. intermedium was redesignated JJ(s)S and that of Th. ponticllm was redesignated JJJJ(s)J(s). The finding of a close relationship among S, J, and J(s) genomes provides valuable markers for molecular cytogenetic analyses using S genome DNA probes to monitor the transfer of useful traits from Th. intermedium and Th. ponticum to wheat.

引用

页码：580 / 586

页数：7

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