Trypsin-catalyzed peptide synthesis with various p-guanidinophenyl esters as acyl donors

被引：0

作者：

Sekizaki, H ^{[1
]}

Itoh, K ^{[1
]}

Toyota, E ^{[1
]}

Tanizawa, K ^{[1
]}

机构：

[1] HLTH SCI UNIV HOKKAIDO, FAC PHARMACEUT SCI, ISHIKARI, HOKKAIDO 06102, JAPAN

来源：

CHEMICAL & PHARMACEUTICAL BULLETIN | 1996年 / 44卷 / 08期

关键词：

trypsin; inverse substrate; peptide synthesis; N-alpha-(tert-butyloxycarbonyl)amino acid p-guanidinophenyl ester; N-alpha-(tert-butyloxycarbonyl)peptide p-guanidinophenyl ester;

D O I：

暂无

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Trypsin-catalyzed peptide synthesis has been studied by using p-guanidinophenyl esters of N-alpha-(tert-butyloxycarbonyl)amino acid and peptide as acyl donor components, The reaction conditions were optimized for organic solvents, pH, and concentration of acceptor, The method was especially useful for the preparation of various peptides containing D-amino acids, The enzymatic hydrolysis of the resulting products was negligible.

引用

页码：1585 / 1587

页数：3

共 17 条

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