Derivatization and chromatographic behavior of the o-phthaldialdehyde amino acid derivatives obtained with various SH-group-containing additives

被引：114

作者：

Molnár-Perl, I ^{[1
]}

机构：

[1] Eotvos Lorand Univ, Inst Inorgan & Analyt Chem, H-1518 Budapest 112, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 913卷 / 1-2期

关键词：

reviews; derivatization; LC; amino acids; OPA derivatives;

D O I：

10.1016/S0021-9673(00)01200-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An overview is presented of HPLC methods currently in use to determine amino acids as their o-phthaldialdyde derivatives in the presence of various SH-group-containing additives. Crucial points that proved to influence the stability of the amino acid OPA derivatives have been discussed in detail: (i) the mol ratios of the OPA-SH-group-containing additive amino acid; (ii) the preparation and storage conditions of the OPA reagents; (iii) the optimum pH conditions for the interactions and elutions; (iv) the behavior of the, believed to be, less stable amino acids, such as glycine, beta -alanine, gamma -aminobutyric acid, histidine, ornithine and lysine. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：283 / 302

页数：20

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