Lipidomics in diabetes and the metabolic syndrome

被引：41

作者：

Gross, Richard W. ^{[1
]}

Han, Xianlin

机构：

[1] Washington Univ, Sch Med, St Louis, MO 63130 USA

[2] Washington Univ, Sch Med, Dept Internal Med, St Louis, MO 63110 USA

[3] Washington Univ, Dept Chem, St Louis, MO 63130 USA

[4] Washington Univ, Sch Med, Dept Mol Biol & Pharmacol, St Louis, MO 63110 USA

来源：

LIPIDOMICS AND BIOACTIVE LIPIDS: SPECIALIZED ANALYTICAL METHODS AND LIPIDS IN DISEASE | 2007年 / 433卷

关键词：

IONIZATION MASS-SPECTROMETRY; BIOLOGICAL SAMPLES; SHOTGUN LIPIDOMICS; CELLULAR LIPIDOMES; CRUDE EXTRACTS; ELECTROSPRAY; TRIACYLGLYCEROLS; QUANTITATION; MYOCARDIUM; MECHANISMS;

D O I：

10.1016/S0076-6879(07)33004-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Shotgun lipidomics, based on multi-dimensional mass spectrometric array analyses after multiplexed sample preparation and intrasource separation, has been recently advanced to a mature technique for the rapid and reproducible global analysis of cellular lipids. At its current stage, this technology enables us to analyze more than 20 lipid classes and thousands of individual lipid molecular species directly from lipid extracts of biologic samples. Following a brief introduction to the foundations underlying this rapidly expanding technology, we present detailed protocols used for the identification and quantitation of plasma triglycerides, determination of the human heart lipidome, and analysis of cellular cardiolipin molecular species. Through the use and practice of shotgun lipidomics, new insights into the cardiovascular pathobiology manifest in diabetes and the metabolic syndrome can be accrued.

引用

页码：73 / 90

页数：18

共 23 条

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