Sequence analysis of a cDNA containing the gag and prot regions of the soybean retrovirus-like element, SIRE-1

被引：9

作者：

Bi, YA ^{[1
]}

Laten, HM ^{[1
]}

机构：

[1] LOYOLA UNIV, DEPT BIOL, CHICAGO, IL 60626 USA

来源：

PLANT MOLECULAR BIOLOGY | 1996年 / 30卷 / 06期

关键词：

aspartic protease; copia-like; gag; LTR; retrotransposon; retrovirus-like; soybean;

D O I：

10.1007/BF00019562

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SIRE-1 is a family of several hundred dispersed copies of a very large DNA element from Glycine max that has features characteristic of retroviruses and retrotransposons. A 2.4 kb SIRE-1-specific fragment was recovered from a soybean cDNA library and sequenced. The sequence contains two ORFs. Theoretical translation of ORF1 produces a gag-prot-like polyprotein containing highly conserved motifs found in retroelement nucleocapsids (CX(2)CX(4)HX(4)C) and aspartic proteases (LDSG). The second ORF is foreshortened. The cDNA also contains nearly 200 bp of a putative 5' LTR just upstream of a tRNA primer-binding site.

引用

页码：1315 / 1319

页数：5