A high-angle neutron fibre diffraction study of the hydration of B-DNA

被引：8

作者：

Shotton, MW

Pope, LH

Forsyth, VT ^{[1
]}

Langan, P

Grimm, H

Rupprecht, A

Denny, RC

Fuller, W

机构：

[1] Univ Keele, Dept Phys, Keele ST5 5BG, Staffs, England

[2] Inst Max Von Laue Paul Langevin, F-38042 Grenoble 9, France

[3] Res Ctr Juelich, Inst Solid State Phys, Julich, Germany

[4] Univ Stockholm, Arrhenius Lab, S-10691 Stockholm, Sweden

[5] Univ London Imperial Coll Sci Technol & Med, Blackett Lab, Biophys Sect, London SW7 2BZ, England

来源：

PHYSICA B-CONDENSED MATTER | 1997年 / 241卷

关键词：

neutron fibre diffraction; hydration; B-DNA;

D O I：

10.1016/S0921-4526(97)00817-X

中图分类号：

O469 [凝聚态物理学];

学科分类号：

070205 ;

摘要：

A high-angle neutron fibre diffraction study of hydration in the B conformation of DNA has been performed on instrument D19 at the Institut Laue-Langevin, Grenoble, using a sample prepared by the 'wet spinning' technique. In common with our previous neutron fibre diffraction studies, this work exploited the ability to isotopically replace H2O in the specimen by D2O. However, in contrast to previous studies, the sample exhibited 'double orientation' so that it was possible to record a three-dimensional diffraction data set. Fourier synthesis techniques were used to image ordered water surrounding the DNA. Two chains of ordered water were identified in the minor groove of the molecule in positions that are consistent with water bridging of sugar O4 and purine N3 or pyrimidine O2 atoms. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：1166 / 1168

页数：3

共 4 条

[1] STRUCTURE OF THE B DNA CATIONIC SHELL AS REVEALED BY AN X-RAY-DIFFRACTION STUDY OF CSDNA - SEQUENCE-SPECIFIC CATIONIC STABILIZATION OF B-FORM DNA [J].