Sonochemical-Assisted Biogenic Synthesis of Theophrasite β-Ni(OH)2 Nanocluster Using Chia Seeds Extract: Characterization and Anticancer Activity

Theophrasite beta-Ni(OH)(2) nanocluster were fabricated via the sonochemical-assisted biogenic method using chia seeds extract as a reducing and stabilizing agent. The optical and morphological feature of the synthesized nanocluster was characterized using UV-Vis, FTIR, FE-SEM-EDS, HR-TEM, DLS, XPS, and XRD analysis. According to FE-SEM and HR-TEM images of the synthesized materials, beta-Ni(OH)(2) nanocluster illustrates the hexagonal particle shape with an average size of 5.8 nm, while the EDS results confirm the high purity of the synthesized nanocluster. Moreover, the XRD pattern of the synthesized materials shows typical peaks that match the reference pattern of the Theophrasite form of beta-Ni(OH)(2) with a hexagonal crystal system. The XPS analysis illustrates that the prepared samples exhibit both Ni2+ and Ni3+ with the predominance of Ni2+ species. Additionally the in-vitro cytotoxic activity of beta-Ni(OH)(2) nanocluster is tested against the MCF7 cell lines (breast cancer cells). The MTT assay results proved that the synthesized beta-Ni(OH)(2) nanocluster has potent cytotoxic activity against breast cancer cell lines (IC50 : 62.7 mu g/mL).