Target Protein Discrimination Based on Array Sensor Using Calcein-Encapsulating Liposomes with Cholesterol by Principal Component Analysis

被引：0

作者：

Imamura, R. ^{[1
]}

Zhang, Z. ^{[1
]}

Yoshikawa, T. ^{[1
]}

Shimanouchi, T. ^{[2
]}

Murata, N. ^{[1
]}

Yamashita, K. ^{[1
]}

Fukuzawa, M. ^{[1
]}

Noda, M. ^{[1
]}

机构：

[1] Kyoto Inst Technol, Sakyo Ku, Kyoto 6068585, Japan

[2] Okayama Univ, Kita Ku, Okayama 7008530, Japan

来源：

EUROSENSORS 2015 | 2015年 / 120卷

关键词：

Biosensor; Fluorescence; Liposome; Protein; Array; Cholesterol; Principal Component Analysis;

D O I：

10.1016/j.proeng.2015.08.760

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

A bio array sensing system is newly composed utilizing different phospholipid liposomes encapsulating fluorescent molecules. We have confirmed a high output intensity of fluorescence emission due to the characteristics dependent on the concentration of fluorescent molecules when the fluorescent molecules leak from inside of the liposome through perturbed lipid membrane. After measuring a whole array image of fluorescence emission output from every element of liposome sensor by a new CMOS imager system, the outputs of fluorescence emission from all the elements were analyzed by a statistical method of principal component analysis (PCA). It was found from obtained PCA plots that different species of proteins with several concentrations were clearly discriminated with high cumulative contribution ratio. (C) 2015 Published by Elsevier Ltd.

引用

页码：699 / 702

页数：4

共 8 条

[1] Discrimination of Target Proteins Using Arrayed Fluorescent Liposomes Incorporated with Cholesterol by Principal Component Analysis
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[2] A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by Principal Component Analysis
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