Detection of 70 kDa heat shock protein in the saliva of dairy cows

被引：12

作者：

Lamy, Elsa ^{[1
]}

Jurkovich, Viktor ^{[2
]}

Rodrigues, Lenia ^{[1
]}

Geraldo, Ana ^{[1
]}

Cachucho, Liliana ^{[1
]}

Silva, Flavio ^{[1
]}

Matos, Catarina ^{[1
]}

Capela e Silva, Fernando ^{[1
,3
]}

Pinheiro, Cristina ^{[1
,4
]}

Konyves, Laszlo ^{[2
]}

Bakony, Mikolt ^{[2
]}

Pereira, Alfredo ^{[1
,4
]}

机构：

[1] Univ Evora, Inst Mediterranean Agr & Environm Sci, Nucleo Mitra, Apartado 94, P-7006554 Evora, Portugal

[2] Univ Vet Med, Dept Anim Hyg Herd Hlth & Vet Ethol, Istvan Utca 2, H-1078 Budapest, Hungary

[3] Univ Evora, Dept Biol, Largo Colegiais 2, P-7000 Evora, Portugal

[4] Univ Evora, Dept Zootech, Largo Colegiais 2, P-7000 Evora, Portugal

来源：

JOURNAL OF DAIRY RESEARCH | 2017年 / 84卷 / 03期

关键词：

heat shock proteins; dairy cow; saliva; HEAT-SHOCK-PROTEIN-70; STRESS;

D O I：

10.1017/S0022029917000280

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

This Research Communication describes, for the first time, the detection of HSP70 in saliva of dairy cows. Thermal stress is a major environmental stress that limits animal growth, metabolism, and productivity. The cellular response to heat stress involves the synthesis of heat shock proteins (HSPs), presumably to protect the functional stability of cells at increasing temperatures. HSP70 has been found to be present in cattle blood serum and may also be present in other secretory fluids, such as saliva, as already observed in humans. The aim of this study was to detect heat shock protein HSP70 in bovine saliva. Saliva samples were taken from higher- (n = 5) and lower milk producing (n = 5) Holstein-Friesian cows in summer and in winter for the detection of HSP70. HSP70 concentrations were assayed using the ELISA technique. Salivary HSP70 concentrations ranged from 0.524 to 12.174 ng/ml in cows. Higher salivary HSP70 concentrations were significantly associated with higher milk production and higher environmental temperature, but not with rectal temperature.

引用

页码：280 / 282

页数：3

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