Bergenin Plays an Anti-Inflammatory Role via the Modulation of MAPK and NF-κB Signaling Pathways in a Mouse Model of LPS-Induced Mastitis

被引：100
作者：
Gao, Xue-jiao [1 ]
Guo, Meng-yao [2 ]
Zhang, Ze-cai [1 ]
Wang, Tian-cheng [1 ]
Cao, Yong-guo [1 ]
Zhang, Nai-sheng [3 ]
机构：
[1] Jilin Univ, Coll Vet Med, Changchun 130062, Jilin Province, Peoples R China
[2] Huazhong Agr Univ, Coll Vet Med, Wuhan 430070, Peoples R China
[3] Jilin Univ, Coll Vet Med, Dept Clin Vet Med, Changchun 130062, Jilin Province, Peoples R China
来源：
INFLAMMATION | 2015年 / 38卷 / 03期
基金：
高等学校博士学科点专项科研基金; 中国国家自然科学基金;
关键词：
bergenin; mastitis; anti-inflammation; nuclear factor-kappaB (NF-kappa B); mitogen-activated protein kinases (MAPKs); LIPOPOLYSACCHARIDE-INDUCED MASTITIS; ACUTE LUNG INJURY; STAPHYLOCOCCUS-AUREUS; ESCHERICHIA-COLI; DOWN-REGULATION; ANTIOXIDANT; DERIVATIVES; ACTIVATION; EXPRESSION; IL-1-BETA;
D O I：
10.1007/s10753-014-0079-8
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Mastitis is a major disease in humans and other animals and is characterized by mammary gland inflammation. It is a major disease of the dairy industry. Bergenin is an active constituent of the plants of genus Bergenia. Research indicates that bergenin has multiple biological activities, including anti-inflammatory and immunomodulatory properties. The objective of this study was to evaluate the protective effects and mechanism of bergenin on the mammary glands during lipopolysaccharide (LPS)-induced mastitis. In this study, mice were treated with LPS to induce mammary gland mastitis as a model for the disease. Bergenin treatment was initiated after LPS stimulation for 24 h. The results indicated that bergenin attenuated inflammatory cell infiltration and decreased the concentration of NO, TNF-alpha, IL1 beta, and IL-6, which were increased in LPS-induced mouse mastitis. Furthermore, bergenin down-regulated the phosphorylation of nuclear factor-kappaB (NF-kappa B) and mitogen-activated protein kinases (MAPK) signaling pathway proteins in mammary glands with mastitis. In conclusion, bergenin reduced the expression of NO, TNF-alpha, IL-1 beta, and IL-6 proinflammatory cytokines by inhibiting the activation of the NF-kappa B and MAPKs signaling pathways, and it may represent a novel treatment strategy for mastitis.
引用
收藏
页码：1142 / 1150
页数：9
相关论文
共 39 条
[1]   The pulmonary physician in critical care. 5: Acute lung injury and the acute respiratory distress syndrome: definitions and epidemiology [J].
Atabai, K ;
Matthay, MA .
THORAX, 2002, 57 (05) :452-458
[2]   Subclinical mastitis causes alterations in nitric oxide, total oxidant and antioxidant capacity in cow milk [J].
Atakisi, Onur ;
Oral, Hasan ;
Atakisi, Emine ;
Merhan, Oguz ;
Pancarci, S. Metin ;
Ozcan, Ayla ;
Marasli, Saban ;
Polat, Bulent ;
Colak, Armagan ;
Kaya, Semra .
RESEARCH IN VETERINARY SCIENCE, 2010, 89 (01) :10-13
[3]   Bovine interleukin-1 expression by cultured mammary epithelial cells (MAC-T) and its involvement in the release of MAC-T derived interleukin-8 [J].
Boudjellab, N ;
Chan-Tang, HS ;
Zhao, X .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 2000, 127 (02) :191-199
[4]   Helenalin reduces Staphylococcus aureus infection in vitro and in vivo [J].
Boulanger, D. ;
Brouillette, E. ;
Jaspar, F. ;
Malouin, F. ;
Mainil, J. ;
Bureau, F. ;
Lekeux, P. .
VETERINARY MICROBIOLOGY, 2007, 119 (2-4) :330-338
[5]   Bovine mastitis: An evolving disease [J].
Bradley, AJ .
VETERINARY JOURNAL, 2002, 164 (02) :116-128
[6]   Antioxidant activity of (+)-bergenin -: a phytoconstituent isolated from the bark of Sacoglottis uchi Huber (Humireaceae) [J].
De Abreu, Heitor A. ;
Lago, Izandina Aparecida dos S. ;
Souza, Gilmar P. ;
Pilo-Veloso, Dorila ;
Duarte, Helio A. ;
Alcantara, Antonio Flavio de C. .
ORGANIC & BIOMOLECULAR CHEMISTRY, 2008, 6 (15) :2713-2718
[7]   Downregulation of NO and PGE2 in LPS-stimulated BV2 microglial cells by trans-isoferulic acid via suppression of PI3K/Akt-dependent NF-κB and activation of Nrf2-mediated HO-1 [J].
Dilshara, Matharage Gayani ;
Lee, Kyoung-Tae ;
Jayasooriya, Rajapaksha Gedara Prasad Tharanga ;
Kang, Chang-Hee ;
Park, Sang Rul ;
Choi, Yung Hyun ;
Choi, Il-Whan ;
Hyun, Jin-Won ;
Chang, Weon-Young ;
Kim, Yeon-Su ;
Lee, Hak-Ju ;
Kim, Gi-Young .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2014, 18 (01) :203-211
[8]  
Ejaz Ahmed Ejaz Ahmed, 2004, Asian Journal of Plant Sciences, V3, P410
[9]   Lipopolysaccharide increases Toll-like receptor 4 and downstream Toll-like receptor signaling molecules expression in bovine endometrial epithelial cells [J].
Fu Yunhe ;
Liu Bo ;
Feng Xiaosheng ;
Liu Zhicheng ;
Liang Dejie ;
Li Fengyang ;
Li Depeng ;
Cao Yongguo ;
Feng Shuang ;
Zhang Xichen ;
Zhang Naisheng ;
Yang Zhengtao .
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 2013, 151 (1-2) :20-27
[10]   Geniposide, from Gardenia jasminoides Ellis, inhibits the inflammatory response in the primary mouse macrophages and mouse models [J].
Fu, Yunhe ;
Liu, Bo ;
Liu, Jinhua ;
Liu, Zhicheng ;
Liang, Dejie ;
Li, Fengyang ;
Li, Depeng ;
Cao, Yongguo ;
Zhang, Xichen ;
Zhang, Naisheng ;
Yang, Zhengtao .
INTERNATIONAL IMMUNOPHARMACOLOGY, 2012, 14 (04) :792-798
1234