High prevalence of Salmonella enterica subsp diarizonae in tonsils of sheep at slaughter

被引：34

作者：

Bonke, R. ^{[2
]}

Wacheck, S. ^{[2
]}

Bumann, C. ^{[2
]}

Thum, C. ^{[2
]}

Stueber, E. ^{[2
]}

Koenig, M. ^{[3
]}

Stephan, R. ^{[4
]}

Fredriksson-Ahomaa, M. ^{[1
]}

机构：

[1] Univ Helsinki, Fac Vet Med, Dept Food Hyg & Environm Hlth, FIN-00014 Helsinki, Finland

[2] Univ Munich, Fac Vet Med, Inst Food Hyg, Munich, Germany

[3] Serv Consommat & Affaires Vet, Geneva, Switzerland

[4] Univ Zurich, Vetsuisse Fac, Inst Food Safety & Hyg, Zurich, Switzerland

来源：

FOOD RESEARCH INTERNATIONAL | 2012年 / 45卷 / 02期

关键词：

Salmonella enterica subsp diarizonae; Small ruminants; Tonsils; PCR; Isolation; PRESUMPTIVE IDENTIFICATION; ESCHERICHIA-COLI; CAMPYLOBACTER; ARIZONAE; GOATS;

D O I：

10.1016/j.foodres.2011.01.050

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The presence of Salmonella was studied in tonsils and feces of sheep and goats at slaughter using PCR and culturing. The isolates were further characterized using PFGE to get more information about the genetic diversity of Salmonella strains circulating among sheep and goats. Antimicrobial resistance was studied because resistance to multiple antimicrobial agents among Salmonella is increasing. The prevalence of Salmonella was 43% and 2% in the tonsils of sheep and goat, respectively. Salmonella was not detected in the feces of adult animals and only sporadically in the feces of juveniles (2%). S. enterica subsp. diarizonae 61: k:1,5,(7) was isolated from 20% of the sheep tonsils and 1% of the goat tonsils. In total, 9 genotypes were obtained with PFGE using SpeI, XbaI, NotI and XhoI restriction enzymes; however, one genotype was predominant. All strains were sensitive to most (13/16) of the antimicrobials. Resistance to sulfamethoxazole was high (95%). Three (15%) strains, which were isolated from lambs, were also resistant to colistin. No correlation between the antimicrobial resistance pattern and the genotype was noticed. These results demonstrate that slaughtered sheep are an important reservoir for S. enterica subsp. diarizonae 61:k:1,5,(7) carrying this pathogen frequently in the tonsils. Future studies are needed to elucidate the significance of the tonsils in the contamination of sheep carcasses and meat with Salmonella. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：880 / 884

页数：5

共 27 条

[1] SALMONELLA IDENTIFICATION BY THE POLYMERASE CHAIN-REACTION [J].

AABO, S ;

RASMUSSEN, OF ;

ROSSEN, L ;

SORENSEN, PD ;

OLSEN, JE .

MOLECULAR AND CELLULAR PROBES, 1993, 7 (03) :171-178

[2] Molecular epidemiology and population genetics of Salmonella subspecies diarizonae in sheep in Norway and Sweden [J].

Alvseike, O ;

Vardund, T ;

Lindstedt, B ;

Heir, E ;

Eriksson, E ;

Kapperud, G .

EPIDEMIOLOGY AND INFECTION, 2004, 132 (02) :253-261

[3] Prevalence of a Salmonella subspecies diarizonae in Norwegian sheep herds [J].

Alvseike, O ;

Skjerve, E .

PREVENTIVE VETERINARY MEDICINE, 2002, 52 (3-4) :277-285

[4]

[Anonymous], 2010, EFSA J, V1, P1658

[5] A recurring salmonellosis epidemic in New Zealand linked to contact with sheep [J].

Baker, M. G. ;

Thornley, C. N. ;

Lopez, L. D. ;

Garrett, N. K. ;

Nicol, C. M. .

EPIDEMIOLOGY AND INFECTION, 2007, 135 (01) :76-83

[6] Isolation of Salmonella from environmental samples collected in the reptile department of Antwerp Zoo using different selective methods [J].

Bauwens, L. ;

Vercammen, F. ;

Bertrand, S. ;

Collard, J. -M. ;

De Ceuster, S. .

JOURNAL OF APPLIED MICROBIOLOGY, 2006, 101 (02) :284-289

[7] Disk prediffusion is a reliable method for testing colistin susceptibility in porcine E. coli strains [J].

Boyen, F. ;

Vangroenweghe, F. ;

Butaye, P. ;

De Graef, E. ;

Castryck, F. ;

Heylen, P. ;

Vanrobaeys, M. ;

Haesebrouck, F. .

VETERINARY MICROBIOLOGY, 2010, 144 (3-4) :359-362

[8]

CHONG Y, 1991, Yonsei Medical Journal, V32, P275

[9]

CLSI. Clinical and Laboratory Standards Institute, 2008, M27A3 CLSI, V28, P13

[10] Increase in Salmonella enterica subspecies diarizonae serovar 61:k:1,5,(7) in sheep [J].

Davies, RH ;

Evans, SJ ;

Preece, BE ;

Chappell, S ;

Kidd, S ;

Jones, YE .

VETERINARY RECORD, 2001, 149 (18) :555-557

← 1 2 3 →