Antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava']java leaf extracts against methicillin-resistant Staphylococcus aureus

被引:48
作者
Chakraborty, Shohini [1 ]
Afaq, Nashra [1 ]
Singh, Neelam [1 ]
Majumdar, Sukanta [2 ]
机构
[1] Saaii Coll Med Sci & Technol, Dept Microbiol, Kanpur 209203, Uttar Pradesh, India
[2] Univ Gour Banga, Dept Bot, Microbiol & Microbial Biotechnol Lab, Malda 732103, W Bengal, India
来源
JOURNAL OF INTEGRATIVE MEDICINE-JIM | 2018年 / 16卷 / 05期
关键词
Methicillin-resistant Staphylococcus aureus; Antimicrobial activity; Plant extracts; Synergistic effect; High-performance thin-layer; chromatography; ESSENTIAL OILS; PREVALENCE; SUSCEPTIBILITY; BACTERIA;
D O I
10.1016/j.joim.2018.07.005
中图分类号
R [医药、卫生];
学科分类号
10 ;
摘要
Objective: This study examined the antimicrobial activity of Cannabis sativa, Thuja orientalis and Psidium guajava against methicillin-resistant Staphylococcus aureus (MRSA) and used a standardized purification protocol to determine the presence and abundance of bioactive compounds in the leaf extracts. Methods: In vitro antimicrobial activities of the ethanolic extracts of C. sativa, T. orientalis and P. guajava were tested against MRSA. The presence of bioactive molecules in these three leaves was evaluated using biochemical assays and high-performance thin-layer chromatography (HPTLC). Results: Resistance to methicillin, penicillin, oxacillin and cefoxitin was observed in each of the clinical and nonclinical MRSA isolates. However, they were still vulnerable to vancomycin. Used individually, the 50% extract of each plant leaf inhibited MRSA growth. A profound synergism was observed when C. sativa was used in combination with T. orientalis (1:1) and when P. guajava was used in combination with T. orientalis (1:1). This was shown by larger zones of inhibition. This synergism was probably due to the combined inhibitory effect of phenolics present in the leaf extracts (i.e., quercetin and gallic acid) and catechin, as detected by HPTLC. Conclusion: The leaf extracts of C. sativa, T. orientalis and P. guajava had potential for the control of both hospital-and community-acquired MRSA. Moreover, the inhibitory effect was enhanced when extracts were used in combination. (C) 2018 Shanghai Changhai Hospital. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:350 / 357
页数:8
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