Autophagy regulates the cell cycle of murine HSPCs in a nutrient-dependent manner

被引：28

作者：

Cao, Yan ^{[1
]}

Zhang, Aihong ^{[1
]}

Cai, Jinyang ^{[1
]}

Yuan, Na ^{[1
]}

Lin, Weiwei ^{[1
]}

Liu, Shengbing ^{[1
]}

Xu, Fei ^{[1
]}

Song, Lin ^{[1
]}

Li, Xin ^{[1
]}

Fang, Yixuan ^{[1
]}

Wang, Zhen ^{[1
]}

Wang, Zhijian ^{[1
]}

Wang, Jian ^{[1
]}

Zhang, Han ^{[1
]}

Zhao, Wenli ^{[1
]}

Hu, Shaoyan ^{[1
]}

Zhang, Suping ^{[1
]}

Wang, Jianrong ^{[1
]}

机构：

[1] Soochow Univ, Collaborat Innovat Ctr Hematol,Sch Med, Jiangsu Key Lab Stem Cell Res,Affiliated Children, Hematol Ctr,Cyrus Tang Med Inst,Jiangsu Inst Hema, Suzhou 215123, Peoples R China

来源：

EXPERIMENTAL HEMATOLOGY | 2015年 / 43卷 / 03期

基金：

中国国家自然科学基金;

关键词：

HEMATOPOIETIC STEM-CELLS; PROGENITOR CELLS; SELF-RENEWAL; BECLIN; QUIESCENCE; PROLIFERATION; HOMEOSTASIS; RETENTION; KINETICS; REVEALS;

D O I：

10.1016/j.exphem.2014.11.002

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Autophagy is implicated in hematopoiesis, but its role in the regulation of the hematopoietic stem and progenitor cell (HSPC) cycle remains obscure. Here, we show that autophagy is essential to maintain and regulate the cell cycle of HSPCs in a nutrient-dependent manner. The loss of autophagy via conditional deletion of its essential gene atg7 ablated the cell cycle of HSPCs. Under physiologic or nutrient-rich conditions, the in vivo activation of autophagy promoted the cell cycle entry of hematopoietic stem cells and upregulated cyclin D3 expression in HSPCs. In contrast, under poor nutrient conditions, the ex vivo inhibition of early, but not late, autophagy signaling events enhanced the G1/S transition of HSPCs. Cyclin D3 was downregulated in HSPCs in response to nutrient stress. A knockdown of cyclin D3 blocked the G1/S transition in HSPCs. Surprisingly, the ex vivo inhibition of starvationinduced early, but not late, autophagy signaling inhibited the ubiquitin-mediated degradation of cyclin D3 in HSPCs. Furthermore, a conditional autophagy defect also had this effect, leading to an elevated cyclin D3 level in these cells. This suggested a dependency of the proteasomal degradation of cyclin D3 on early, but not late, events in autophagy signaling under nutrient stress in HSPCs. Our results thus indicate a dual role of autophagy in the nutrient-dependent modulation of the cell cycle entry of hematopoietic stem cells and G1/S transition of HSPCs via the regulation of cyclin D3 to maintain a proper cell cycle in HSPCs and normal hematopoiesis during adult life. Copyright (C) 2015 ISEH - International Society for Experimental Hematology. Published by Elsevier Inc.

引用

页码：229 / 242

页数：14

共 43 条

[41] Pten dependence distinguishes haematopoietic stem cells from leukaemia-initiating cells [J].