Randomly amplified polymorphic DNA (RAPD) profiles for the distinction of Lactobacillus species

被引:22
作者
Nigatu, A
Ahrné, S
Molin, G
机构
[1] Ethiopian Hlth & Nutr Res Inst, Addis Ababa, Ethiopia
[2] Lund Univ, Ctr Chem, Dept Food Technol, Lab Food Hyg, S-22100 Lund, Sweden
来源
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 2001年 / 79卷 / 01期
关键词
identification; Lactobacillus; RAPD; taxonomy; type strains;
D O I
10.1023/A:1010290403124
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Forty-one type and reference strains of Lactobacillus were evaluated using their randomly amplified polymorphic DNA band profiles. Developed bands for each strain were distinct and enabled discrimination. The best correlations were obtained applying the Pearson product moment correlation coefficient (r) together with the unweighted pair group method using arithmetic averages algorithm. All of the strains were clearly differentiated at and below the 72% similarity value. Species discrimination might be possible making use of the distinctly polymorphic bands amplified specific to a strain.
引用
收藏
页码:1 / 6
页数:6
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