bacterial contamination;
organ culture;
human corneas;
D O I:
10.1097/00003226-200104000-00005
中图分类号:
R77 [眼科学];
学科分类号:
100212 ;
摘要:
Purpose. The aim of this study was to detect and identify bacterial contaminants in human corneoscleral tissue after organ culture storage. Methods. Seventy-two corneoscleral rims and corneal buttons trephined from organ cultured corneoscleral discs using aseptic technique and 45 organ-cultured donor corneoscleral rims postpenetrating keratoplasty) were subjected to a mechanical extraction technique using a Stomacher laboratory blender. As a control, 28 of the corneoscleral rims and buttons were halved; one half of each corneoscleral rim and button was decontaminated in formalin for 48 hours before thorough washing in balanced salt solution. Corneal specimens, culture medium, and transport (5% dextran) medium were cultured in brain-heart infusion broth at 37 degreesC for 5 days. Bacterial isolates were identified after culture of turbid enrichment broth. Results. Bacterial contamination was demonstrated in 29% (21 of 72) of the corneoscleral rims and 15% (11 of 72) of the corneal buttons that were trephined aseptically from corneoscleral discs and in 29% (13 of 45) of postkeratoplasty corneoscleral rims. Bacterial contaminants were not isolated from controls. Isolated microorganisms included coagulase-negative Staphylococcus, Staphylococcus aureus, Streptococcus viridans, Pseudomonas sp, and Bacillus sp. A correlation was not demonstrated between contamination and cause of death, death to enucleation, death to culture time, or time in culture. Postkeratoplasty endophthalmitis was not evident in the patients who had received corneal buttons from those corneoscleral discs that had contaminated corneoscleral rims. Conclusions. Bacterial contamination exists in corneoscleral tissue after organ culture storage. The difference in distribution of bacteria and percentage of contamination between the peripheral and central corneas causes us to question the value of routine postpenetrating keratoplasty corneoscleral rim cultures.
机构:
Wayne State Univ, Kresge Eye Inst, Sch Med, Dept Ophthalmol, Detroit, MI 48201 USA
Wayne State Univ, Kresge Eye Inst, Sch Med, Dept Anat & Cell Biol, Detroit, MI 48201 USAWayne State Univ, Kresge Eye Inst, Sch Med, Dept Ophthalmol, Detroit, MI 48201 USA
Gao, Nan
Mcdermott, Mark
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机构:
Wayne State Univ, Kresge Eye Inst, Sch Med, Dept Ophthalmol, Detroit, MI 48201 USA
Wayne State Univ, Kresge Eye Inst, Sch Med, Dept Anat & Cell Biol, Detroit, MI 48201 USAWayne State Univ, Kresge Eye Inst, Sch Med, Dept Ophthalmol, Detroit, MI 48201 USA
Mcdermott, Mark
Yu, Fu-Shin
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h-index: 0
机构:
Wayne State Univ, Kresge Eye Inst, Sch Med, Dept Ophthalmol, Detroit, MI 48201 USA
Wayne State Univ, Kresge Eye Inst, Sch Med, Dept Anat & Cell Biol, Detroit, MI 48201 USAWayne State Univ, Kresge Eye Inst, Sch Med, Dept Ophthalmol, Detroit, MI 48201 USA
Yu, Fu-Shin
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS,
2025,
(215):
机构:
Cedars Sinai Med Ctr, Eye Program, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
Cedars Sinai Med Ctr, Dept Neurosurg, Los Angeles, CA 90048 USA
Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA
Univ Calif Los Angeles, Los Angeles, CA USACedars Sinai Med Ctr, Eye Program, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
Saghizadeh, Mehrnoosh
Dib, Christian M.
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h-index: 0
机构:
Univ Calif Los Angeles, Los Angeles, CA USACedars Sinai Med Ctr, Eye Program, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
Dib, Christian M.
Brunken, William J.
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h-index: 0
机构:
SUNY Upstate Med Univ, Dept Ophthalmol, Ctr Vis Res, Syracuse, NY 13210 USA
SUNY Upstate Med Univ, Dept Neurosci & Physiol, Syracuse, NY 13210 USACedars Sinai Med Ctr, Eye Program, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
Brunken, William J.
Ljubimov, Alexander V.
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h-index: 0
机构:
Cedars Sinai Med Ctr, Eye Program, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
Cedars Sinai Med Ctr, Dept Neurosurg, Los Angeles, CA 90048 USA
Cedars Sinai Med Ctr, Dept Biomed Sci, Los Angeles, CA 90048 USA
Univ Calif Los Angeles, Los Angeles, CA USACedars Sinai Med Ctr, Eye Program, Board Governors Regenerat Med Inst, Los Angeles, CA 90048 USA
机构:
Cedars Sinai Med Ctr, Regenerat Med Inst, Los Angeles, CA 90048 USA
Univ Calif Los Angeles, Med, Los Angeles, CA USACedars Sinai Med Ctr, Regenerat Med Inst, Los Angeles, CA 90048 USA
Ljubimov, Alexander V.
Dib, Christian M.
论文数: 0引用数: 0
h-index: 0
机构:
Univ Calif Los Angeles, Med, Los Angeles, CA USACedars Sinai Med Ctr, Regenerat Med Inst, Los Angeles, CA 90048 USA
Dib, Christian M.
Saghizadeh, Mehrnoosh
论文数: 0引用数: 0
h-index: 0
机构:
Cedars Sinai Med Ctr, Regenerat Med Inst, Los Angeles, CA 90048 USACedars Sinai Med Ctr, Regenerat Med Inst, Los Angeles, CA 90048 USA