Lipidomic profiling of extracellular vesicles derived from prostate and prostate cancer cell lines

被引：125

作者：

Brzozowski, Joshua S. ^{[1
,2
]}

Jankowski, Helen ^{[1
,2
]}

Bond, Danielle R. ^{[1
,2
]}

McCague, Siobhan B. ^{[1
,2
]}

Munro, Benjamin R. ^{[2
,3
]}

Predebon, Melanie J. ^{[2
,3
]}

Scarlett, Christopher J. ^{[2
,3
]}

Skelding, Kathryn A. ^{[2
,4
]}

Weidenhofer, Judith ^{[1
,2
]}

机构：

[1] Univ Newcastle, Sch Biomed Sci & Pharm, Ourimbah, NSW, Australia

[2] Hunter Med Res Inst, Canc Res Program, New Lambton Hts, NSW, Australia

[3] Univ Newcastle, Sch Environm & Life Sci, Ourimbah, NSW, Australia

[4] Univ Newcastle, Sch Biomed Sci & Pharm, Callaghan, NSW, Australia

来源：

LIPIDS IN HEALTH AND DISEASE | 2018年 / 17卷

关键词：

Extracellular vesicles; Lipidomics; Prostate cancer; URINARY EXOSOMES; BIOMARKERS; CLASSIFICATION; ACCUMULATION; METABOLISM; RELEASE;

D O I：

10.1186/s12944-018-0854-x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Extracellular vesicles (EVs) are produced and secreted from most cells of the body and can be recovered in biological fluids. Although there has been extensive characterisation of the protein and nucleic acid component of EVs, their lipidome has received little attention and may represent a unique and untapped source of biomarkers for prostate cancer diagnosis and prognosis. Methods: EVs were isolated from non-tumourigenic (RWPE1), tumourigenic (NB26) and metastatic (PC-3) prostate cell lines. Lipids were extracted and subsequently used for targeted lipidomics analysis for the quantitation of molecular lipid species. Results: A total of 187 molecular lipid species were quantitatively identified in EV samples showing differential abundance between RWPE1, NB26 and PC-3 EV samples. Fatty acids, glycerolipids and prenol lipids were more highly abundant in EVs from non-tumourigenic cells, whereas sterol lipids, sphingolipids and glycerophospholipids were more highly abundant in EVs from tumourigenic or metastatic cells. Conclusions: This study identified differences in the molecular lipid species of prostate cell-derived EVs, increasing our understanding of the changes that occur to the EV lipidome during prostate cancer progression. These differences highlight the importance of characterising the EV lipidome, which may lead to improved diagnostic and prognostic biomarkers for prostate cancer.

引用

页数：12

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