Agrin induces association of Chrna1 mRNA and nicotinic acetylcholine receptor in C2C12 myotubes

被引：10
作者：
Chang, Yung-Fu [2 ]
Chou, Hui-Ju [2 ]
Yen, Ying-Cheng [2 ]
Chang, Hsueh-Wei [2 ]
Hong, Yi-Ren [4 ]
Huang, Hurng-Wern [1 ,5 ]
Tseng, Chao-Neng [1 ,2 ,3 ]
机构：
[1] Kaohsiung Med Univ, Grad Inst Nat Prod, Kaohsiung 807, Taiwan
[2] Kaohsiung Med Univ, Dept Biomed Sci & Environm Biol, Kaohsiung 807, Taiwan
[3] Kaohsiung Med Univ, Ctr Canc, Kaohsiung Med Univ Hosp, Kaohsiung 807, Taiwan
[4] Kaohsiung Med Univ, Grad Inst Biochem, Kaohsiung 807, Taiwan
[5] Natl Sun Yat Sen Univ, Inst Biomed Sci, Kaohsiung 804, Taiwan
来源：
FEBS LETTERS | 2012年 / 586卷 / 19期
关键词：
Neuromuscular junction; mRNA localization; mRNA targeting; Synapse; AChR; Chrna1; Agrin; Staufen; NEUROMUSCULAR-JUNCTION; PROTEIN-SYNTHESIS; SKELETAL-MUSCLE; GENE-EXPRESSION; TRANSLATIONAL CONTROL; SYNAPTIC SITES; DENDRITES; LOCALIZATION; STAUFEN1; NEURONS;
D O I：
10.1016/j.febslet.2012.07.068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In the mammalian central nervous system transcripts of certain synaptic components are localized near the synapse, allowing for rapid regulation of protein levels. Here we test whether an mRNA localization mechanism also exists in the postsynaptic specialization induced by agrin in C2C12 myotubes. RT-PCR showed that Chrna1 was co-purified with nicotinic acetylcholine receptor (AChR) isolated by affinity column or by ultracentrifugation. In addition, Stau1 was found to interact with Chrna1 mRNA, and knocking down of Stau1 by RNAi resulted in defective AChR clustering. These results suggest that mRNA localization also participates in the formation of mammalian neuromuscular junction (NMJ). Structured summary of protein interactions: AChR alpha 1 physically interacts with Stau1 by pull down (View interaction) (C) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：3111 / 3116
页数：6
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