Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein

被引：135

作者：

Kumar, N. S. Sampath ^{[1
]}

Nazeer, R. A. ^{[1
]}

Jaiganesh, R. ^{[1
]}

机构：

[1] SRM Univ, Dept Biotechnol, Sch Bioengn, Kattankulathur 603203, Tamil Nadu, India

来源：

PEPTIDES | 2011年 / 32卷 / 07期

关键词：

Horse mackerel; Antioxidant peptide; In vitro gastrointestinal; ESR spectrometer; Lipid peroxidation; OXIDATIVE STRESS; FRAME PROTEIN; DISEASE;

D O I：

10.1016/j.peptides.2011.05.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the present study, a peptide having high antioxidant properties was isolated from horse mackerel viscera protein, Magalaspis cordyla. In vitro gastrointestinal digestion was employed to obtain potential protein hydrolysate and was subjected to consecutive chromatographic methods using fast protein liquid chromatography (FPLC) connected to diethyl amino ethyl (DEAE) anion exchange column and Sephadex G-25 gel filtration column. The activity of the fractions was tested against DPPH and hydroxyl radicals and the isolated peptide showed 89.2 and 59.1 percentage of scavenging. The amino acid sequence of purified peptide was determined using ESI-MS/MS as Ala-Cys-Phe-Leu (518.5 Da), it exhibited high activity against polyunsaturated fatty acid (PUFA) peroxidation than that of natural antioxidant, alpha-tocopherol. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：1496 / 1501

页数：6

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