The Receptor-like Kinase TaCRK-7A Inhibits Fusarium pseudograminearum Growth and Mediates Resistance to Fusarium Crown Rot in Wheat

被引：10

作者：

Wu, Tianci ^{[1
,2
]}

Guo, Feilong ^{[1
,2
]}

Xu, Gangbiao ^{[2
]}

Yu, Jinfeng ^{[3
]}

Zhang, Li ^{[3
]}

Wei, Xuening ^{[1
]}

Zhu, Xiuliang ^{[1
]}

Zhang, Zengyan ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China

[2] Cent South Univ Forestry & Technol, Lab Forestry Genet, Changsha 410004, Peoples R China

[3] Shandong Agr Univ, Coll Plant Protect, Tai An 271018, Shandong, Peoples R China

来源：

BIOLOGY-BASEL | 2021年 / 10卷 / 11期

关键词：

antifungal activity; receptor-like kinase with DUF26 domain-containing; Fusarium crown rot resistance; Fusarium pseudograminearum; wheat (Triticum aestivum); PROTEIN; DEFENSE; IDENTIFICATION;

D O I：

10.3390/biology10111122

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Simple SummaryFusarium crown rot (FCR), caused by a soil-borne fungus Fusarium pseudograminearum, is one of the most destructive diseases of cereal crops, including wheat (Triticum aestivum) in many countries. It is vital to isolate resistance genes for improving crop resistance. Herein, we report the positive function of the wheat DUF26 domain-containing receptor-like kinase TaCRK-7A in the host resistance response to the pathogen F. pseudograminearum attack. The purified TaCRK-7A protein directly inhibited F. pseudograminearum mycelial growth. The TaCRK-7A transcript was elevated upon F. pseudograminearum infection and the transcript induction was higher in resistant wheat genotypes than in susceptible wheat genotypes. Knocking down of TaCRK-7A compromised resistance of wheat to FCR and significantly reduced the transcript levels of defense genes in wheat. This study provides a novel insight into the wheat immune responses to F. pseudograminearum.The fungus F. pseudograminearum can cause the destructive disease Fusarium crown rot (FCR) of wheat, an important staple crop. Functional roles of FCR resistance genes in wheat are largely unknown. In the current research, we characterized the antifungal activity and positive-regulatory function of the cysteine-rich repeat receptor-like kinase TaCRK-7A in the defense against F. pseudograminearum in wheat. Antifungal assays showed that the purified TaCRK-7A protein inhibited the growth of F. pseudograminearum. TaCRK-7A transcript abundance was elevated after F. pseudograminearum attack and was positively related to FCR-resistance levels of wheat cultivars. Intriguingly, knocking down of TaCRK-7A transcript increased susceptibility of wheat to FCR and decreased transcript levels of defense-marker genes in wheat. Furthermore, the transcript abundances of TaCRK-7A and its modulated-defense genes were responsive to exogenous jasmonate treatment. Taken together, these results suggest that TaCRK-7A can directly inhibit F. pseudograminearum growth and mediates FCR-resistance by promoting the expression of wheat defense genes in the jasmonate pathway. Thus, TaCRK-7A is a potential gene resource in FCR-resistant wheat breeding program.

引用

页数：10