Molecular cloning and characterization of a novel gene, EMILIN-5, and its possible involvement in skeletal development

被引：23
作者：
Doi, M
Nagano, A
Nakamura, Y
机构：
[1] Univ Tokyo, Inst Med Sci, Ctr Human Genome, Lab Mol Med,Minato Ku, Tokyo 1088639, Japan
[2] Hamamatsu Univ Sch Med, Dept Orthopaed Surg, Shizuoka 4313192, Japan
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 313卷 / 04期
基金：
日本学术振兴会;
关键词：
cDNA microarray; bone; EMILIN; mesenchymal stem cells; mineralization; osteogenesis; perichondrium; skeletal development;
D O I：
10.1016/j.bbrc.2003.11.181
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
By analyzing expression profiles of human mesenchymal stem cells incubated in osteogenic supplements, we identified and characterized a novel human cDNA, elastin microfibril interface located protein-5 (EMILIN-5), that is likely to play a significant role in the process of osteogenesis. The deduced EMILIN-5 product consists of 766 amino acids with a cysteine-rich EMI domain at the NH2 terminus. Western blotting detected EMILIN-5 expression in a variety of osteoblastic cell lines. Immunohistochemistry of mouse embryos 13.5 days post-coitus revealed relatively high levels of EMILIN-5 protein in perichondrium cells of developing limbs. Our findings suggest that the EMILIN-5 gene plays an important role in skeletal development. (C) 2003 Elsevier Inc. All rights reserved.
引用
收藏
页码：888 / 893
页数：6
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