Antibacterial, adhesive, and MSC exosomes encapsulated microneedles with spatio-temporal variation functions for diabetic wound healing

被引：72

作者：

Gan, Jingjing ^{[1
]}

Zhang, Xiaoxuan ^{[2
]}

Ma, Wenjuan ^{[1
]}

Zhao, Yuanjin ^{[1
,2
]}

Sun, Lingyun ^{[1
]}

机构：

[1] Nanjing Univ, Med Sch, Affiliated Drum Tower Hosp, Inst Translat Med,Dept Rheumatol & Immunol, Nanjing 210008, Peoples R China

[2] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Peoples R China

来源：

NANO TODAY | 2022年 / 47卷

基金：

中国国家自然科学基金; 美国国家科学基金会;

关键词：

Bioinspired; Microneedle; Wound healing; MSC-exosome; Patch; CHALLENGES; MANAGEMENT; HYDROGEL; OPPORTUNITIES; NANOPARTICLE;

D O I：

10.1016/j.nantod.2022.101630

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Chronic wounds affect healthcare systems and increase the risk of infection. Despite the clinical demand for improving healing, an effective and safe therapeutic approach is still lacking. Here, we present a novel spatio-temporal-varying adhesive microneedle (MN) patch encapsulated with mesenchymal stem cell-derived exosomes (MSC-exos) and antibacterial Ag nanoparticles (AgNPs) for improving wound healing. This MN patch is fabricated by replicating a predesigned flexible template. Consisting of porous metha-crylate gelatin hydrogels, the MN tips can continuously deliver anti-inflammatory and pro-angiogenic MSC-exos to wound beds, which can accelerate healing by improving cell functions, remodeling blood vessels and restoring immune systems. Besides, the silk-fibroin-composed MN back enables the patch to stick to the skin well and fix at the wounded site, thus avoiding secondary bandage. By incorporating AgNPs in the MN back, the MN patch is endowed with the ability to efficiently resist microbial contamination and further benefits wound repair. Such a composite MN patch performs well in relieving inflammation, promoting angiogenesis, and suppressing wound-infecting bacteria during wound injury in diabetic rats, with no adverse effects observed. All the features make this multifunctional MN patch potentially valuable for clinical applications.(c) 2022 Published by Elsevier Ltd.

引用

页数：12

共 48 条

[1] Polymer-based biomaterials for chronic wound management: Promises and challenges [J].

Arif, Muhammad Maaz ;

Khan, Shahzad Maqsood ;

Gull, Nafisa ;

Tabish, Tanveer A. ;

Zia, Saba ;

Khan, Rafi Ullah ;

Awais, Sayed Muhammad ;

Butt, Muhammad Arif .

INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2021, 598

[2] Preventing and treating foot complications associated with diabetes mellitus [J].

Bowling, Frank L. ;

Rashid, S. Tawqeer ;

Boulton, Andrew J. M. .

NATURE REVIEWS ENDOCRINOLOGY, 2015, 11 (10) :606-616

[3] Sprayable hydrogel dressing accelerates wound healing with combined reactive oxygen species-scavenging and antibacterial abilities [J].

Cheng, Hao ;

Shi, Zhe ;

Yue, Kan ;

Huang, Xusheng ;

Xu, Yichuan ;

Gao, Chenghao ;

Yao, Zhongqi ;

Zhang, Yu Shrike ;

Wang, Jian .

ACTA BIOMATERIALIA, 2021, 124 :219-232

[4]

Choi G, 2019, BIOMATER RES, V23

[5] Toxicity, preparation methods and applications of silver nanoparticles: an update [J].

Choudhary, Anuj ;

Singh, Sanjiv ;

Ravichandiran, V .

TOXICOLOGY MECHANISMS AND METHODS, 2022, 32 (09) :650-661

[6] Bio-inspired crosslinking and matrix-drug interactions for advanced wound dressings with long-term antimicrobial activity [J].

Dhand, Chetna ;

Venkatesh, Mayandi ;

Barathi, Veluchami Amutha ;

Harini, Sriram ;

Bairagi, Samiran ;

Leng, Eunice Goh Tze ;

Muruganandham, Nandhakumar ;

Low, Kenny Zhi Wei ;

Fazil, Mobashar Hussain Urf Turabe ;

Loh, Xian Jun ;

Srinivasan, Dinesh Kumar ;

Liu, Shou Ping ;

Beuerman, Roger W. ;

Verma, Navin Kumar ;

Ramakrishna, Seeram ;

Lakshminarayanan, Rajamani .

BIOMATERIALS, 2017, 138 :153-168

[7] Hydrogel-Forming Microneedle Arrays for Enhanced Transdermal Drug Delivery [J].

Donnelly, Ryan F. ;

Singh, Thakur Raghu Raj ;

Garland, Martin J. ;

Migalska, Katarzyna ;

Majithiya, Rita ;

McCrudden, Cian M. ;

Kole, Prashant Laxman ;

Mahmood, Tuan Mazlelaa Tuan ;

McCarthy, Helen O. ;

Woolfson, A. David .

ADVANCED FUNCTIONAL MATERIALS, 2012, 22 (23) :4879-4890

[8] Green Synthesis of Silk Fibroin-Silver Nanoparticle Composites with Effective Antibacterial and Biofilm-Disrupting Properties [J].

Fei, Xiang ;

Jia, Minghui ;

Du, Xin ;

Yang, Yuhong ;

Zhang, Ren ;

Shao, Zhengzhong ;

Zhao, Xia ;

Chen, Xin .

BIOMACROMOLECULES, 2013, 14 (12) :4483-4488

[9] Chronic Wound Healing: A Review of Current Management and Treatments [J].

Han, George ;

Ceilley, Roger .

ADVANCES IN THERAPY, 2017, 34 (03) :599-610

[10] MSC-Derived Exosome Promotes M2 Polarization and Enhances Cutaneous Wound Healing [J].

He, Xiaoning ;

Dong, Zhiwei ;

Cao, Yina ;

Wang, Han ;

Liu, Shiyu ;

Liao, Li ;

Jin, Yan ;

Yuan, Lin ;

Li, Bei .

STEM CELLS INTERNATIONAL, 2019, 2019

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