Studies of polyphenol oxidase inactivation by means of high pressure carbon dioxide (HPCD)

被引：11
作者：
Benito-Roman, Oscar [1 ]
Teresa Sanz, M. [1 ]
Melgosa, Rodrigo [1 ]
de Paz, Esther [1 ]
Escudero, Isabel [1 ]
Beltran, Sagrario [1 ]
机构：
[1] Univ Burgos, Fac Sci, Dept Biotechnol & Food Sci, Chem Engn Sect, Plaza Misael Bafiuelos S-N, Burgos 09001, Spain
来源：
JOURNAL OF SUPERCRITICAL FLUIDS | 2019年 / 147卷
关键词：
Polyphenol oxidase; HPCD; Enzyme inactivation; Supercritical carbon dioxide; THERMAL INACTIVATION; CO2; SOLUBILITY; JUICE; MODEL; DEGRADATION; PEROXIDASE; SECONDARY; PIGMENTS; DENSITY; PROTEIN;
D O I：
10.1016/j.supflu.2018.07.026
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
Tyrosinase from mushroom was used as a model polyphenol oxidase (PPO) enzyme to perform a systematic inactivation study using High Pressure Carbon Dioxide (HPCD). The ratio CO2/volume of enzyme (g/mL) loaded in the reactor was found to be critical. Above a critical ratio, pressure, temperature and time did not control the inactivation performance. Exposure time (2-15 min), temperature (25-45 degrees C) and pressure (5-20 MPa) allowed to show a characteristic inactivation pattern for PPO: a sudden decrease in activity (at least 75% of the total activity loss was observed within the first 2 min) was followed by a slowed decay. The experimental data were fitted into a two fraction kinetic model and the main kinetic parameters (Z(P), Z(T) activation volume and activation energy) were calculated. The fluorescence spectroscopy analysis of the samples treated with HPCD revealed significant changes in the tertiary structure of the enzyme.
引用
收藏
页码：310 / 321
页数：12
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