Evaluation of cytokine profile in cervicovaginal lavage specimens of women having asymptomatic reproductive tract infections

Reproductive tract infections (RTIs) such as vaginal candidiasis and bacterial vaginosis (BV) are common among sexually active women and can be both symptomatic or asymptomatic. The microbiota of the reproductive tract triggers immune response at the cervicovaginal interface resulting in secretion of cytokines during the course of these RTIs. The objective of this study was to evaluate the cytokine profile in cervicovaginal lavage of women having asymptomatic vaginal infections. Measurement of vaginal cytokines was done for various interleukins including IL-1 beta, IL-6, IL-8, IL-10, IL-12/IL23p40, IL-17A, tumour necrosis factor alpha (TNF-alpha) and interferon gamma (IFN-gamma) by ProcartaPlex (TM) Multiplex Immunoassay. Women having vaginal Candida infection had increased concentration of IL-1 beta (p=.01), IL-6 (p=.007), IL-8 (p=.327), IL-12/IL23p40 (p=.049) and IFN-gamma (p=.125). The results of our study suggest that evaluation of these cytokines could be explored as an additional measure to determine host inflammatory response in women having asymptomatic vaginal candidiasis.Impact Statement What is already known on this subject? Studies assessing the vaginal cytokine profile to assess the vaginal milieu in various cohorts such as post-menopausal women, pregnant women, women with history of preterm birth, CIN and scheduled IVF cycle are being undertaken. Variable cytokine response has been reported in literature in women with symptomatic bacterial vaginosis and Candida infection. However, much less is known about vaginal cytokine profile in asymptomatic infection. What do the results of this study add? The results of the study show increased concentration of the pro-inflammatory cytokines IL-1 beta, IL-6 IL-8, IL-12/IL23p40 and interferon gamma (IFN-gamma) in women having asymptomatic Candida, vaginal leucocytosis and raised vaginal pH. What are the implications of these findings for clinical practice and/or further research? Evaluation of vaginal cytokine profile (IL-1 beta, IL-6 IL-8, IL-1 beta, IL-12/IL23p40 and IFN-gamma) could be explored as an additional measure to determine inflammation in asymptomatic women. Vaginal cytokines (IL-1 beta, IL-6 IL-8, IL-1 beta, IL-12/IL23p40 and IFN-gamma) could be used further for development of a point of care test.