Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress

被引：78

作者：

Chitraju, Chandramohan ^{[1
]}

Troetzmueller, Martin ^{[2
]}

Hartler, Juergen ^{[3
,4
]}

Wolinski, Heimo ^{[1
]}

Thallinger, Gerhard G. ^{[3
,4
]}

Lass, Achim ^{[1
]}

Zechner, Rudolf ^{[1
]}

Zimmermann, Robert ^{[1
]}

Koefeler, Harald C. ^{[2
]}

Spener, Friedrich ^{[1
]}

机构：

[1] Graz Univ, Lipid Res Ctr, Dept Mol Biosci, A-8010 Graz, Austria

[2] Med Univ Graz, Lipid Res Ctr, Med Res Ctr, Core Facil Mass Spectrometry, A-8010 Graz, Austria

[3] Graz Univ Technol, Inst Genom & Bioinformat, A-8010 Graz, Austria

[4] Austrian Ctr Ind Biotechnol, Core Facil Bioinformat, A-8010 Graz, Austria

来源：

JOURNAL OF LIPID RESEARCH | 2012年 / 53卷 / 10期

基金：

奥地利科学基金会;

关键词：

lipidomics; mass spectrometry; high-fat diet; fasting; steatosis; principal component analysis; NONALCOHOLIC FATTY LIVER; LOW-DENSITY LIPOPROTEIN; INSULIN-RESISTANCE; MASS-SPECTROMETRY; TRIACYLGLYCEROL; PHOSPHATIDYLCHOLINE; DIACYLGLYCEROL; ACTIVATION; LIPOLYSIS; ETHER;

D O I：

10.1194/jlr.M028902

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Liver steatosis can be induced by fasting or high-fat diet. We investigated by lipidomic analysis whether such metabolic states are reflected in the lipidome of hepatocyte lipid droplets (LDs) from mice fed normal chow diet (FED), fasted (FAS), or fed a high-fat diet (HFD). LC-MS/MS at levels of lipid species profiles and of lipid molecular species uncovered a FAS phenotype of LD enriched in triacylglycerol (TG) molecular species with very long-chain (VLC)-PUFA residues and an HFD phenotype with less unsaturated TG species in addition to characteristic lipid marker species. Nutritional stress did not result in dramatic structural alterations in diacylglycerol (DG) and phospholipid (PL) classes. Moreover, molecular species of bulk TG and of DG indicated concomitant de novo TG synthesis and lipase-catalyzed degradation to be active in LDs. DG species with VLC-PUFA residues would be preferred precursors for phosphatidylcholine (PC) species, the others for TG molecular species. In addition, molecular species of PL classes fitted the hepatocyte Kennedy and phosphatidylethanolamine methyltransferase pathways. We demonstrate that lipidomic analysis of LDs enables phenotyping of nutritional stress. TG species are best suited for such phenotyping, whereas structural analysis of TG, DG, and PL molecular species provides metabolic insights.-Chitraju, C., M. Trotzmuller, J. Hartler, H. Wolinski, G. G. Thallinger, A. Lass, R. Zechner, R. Zimmerman, H. C. Kofeler, and F. Spener. Lipidomic analysis of lipid droplets from murine hepatocytes reveals distinct signatures for nutritional stress. J. Lipid Res. 2012. 53: 2141-2152.

引用

页码：2141 / 2152

页数：12

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