Reverse phase high performance liquid chromatographic (HPLC) method for nimesulide tablets dosage form prepared for in vivo in vitro correlation (IVIVC) studies

The cost effective and simple method of reverse phase high performance liquid chromatographic (HPLC) method was successfully developed for the assaying of nimesulide (NM) in tablets, formulated for in vivo in vitro correlation (IVIVC) studies. The assay consisted of isocratic elution of nimesulide in Hiber RT 254-4.6 Purospher STAR RP-18 column using mobile phase composed of acetonitrile, phosphate buffer (pH 5.5) and methanol in the ratio of 35:45:20, respectively. Nimesulide was detected at 230 nm at the flow rate of 1.4 ml/min. The method was found to be sensitive and linear in the range of 1 to 100 ng/ml with 0.9997 coefficient of correlation. Blank sample of mobile phase and placebo showed no interference with the peak of analyte at retention time of 7.93 min. All validation parameters were evaluated and were found within the acceptance limits. The simplicity and short run time makes the method more convenient for quantitative analysis and assay of tablets.