Microwave-immobilized polybutadiene stationary phase for reversed-phase high-performance liquid chromatography

被引：12

作者：

Lopes, NP ^{[1
]}

Collins, KE ^{[1
]}

Jardim, ICSF ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Quim, LABCROM, Lab Pesquisas & Cromatol Liquida, BR-13084971 Campinas, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1030卷 / 1-2期

关键词：

stationary phases; LC; polybutadiene-silica stationary phases; microwave-assisted immobilization;

D O I：

10.1016/j.chroma.2003.09.029

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Polybutadiene (PBD) has been immobilized on high-performance liquid chromatography (HPLC) silica by microwave radiation at various power levels (52-663 W) and actuation times (3-60 min). Columns prepared from these reversed-phase HPLC materials, as well as from similar non-irradiated materials, were tested with standard sample mixtures and characterized by elemental analysis (%C) and infrared spectroscopy. A microwave irradiation of 20 min at 663 W gives a layer of immobilized PBD that presented good performance. Longer irradiation times give thicker immobilized layers having less favorable chromatographic properties. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：225 / 229

页数：5

共 43 条

[1] Characterization and high performance liquid chromatographic evaluation of a new amide-functionalized reversed phase column [J].