Rapid diversification of coevolving marine Synechococcus and a virus

被引:142
作者
Marston, Marcia F. [2 ]
Pierciey, Francis J., Jr. [2 ]
Shepard, Alicia [2 ]
Gearin, Gary [3 ]
Qi, Ji [4 ]
Yandava, Chandri [3 ]
Schuster, Stephan C. [4 ]
Henn, Matthew R. [3 ]
Martiny, Jennifer B. H. [1 ]
机构
[1] Univ Calif Irvine, Dept Ecol & Evolutionary Biol, Irvine, CA 92697 USA
[2] Roger Williams Univ, Dept Biol & Marine Biol, Bristol, RI 02809 USA
[3] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA
[4] Penn State Univ, Ctr Comparat Genom & Bioinformat, University Pk, PA 16802 USA
基金
美国国家科学基金会;
关键词
cyanophage; bacterial diversity; arms race; ANTAGONISTIC COEVOLUTION; MOLECULAR EVOLUTION; ESCHERICHIA-COLI; PHAGE; BACTERIOPHAGE; RESISTANCE; PROCHLOROCOCCUS; RECOMBINATION; CYANOBACTERIA; CYANOPHAGES;
D O I
10.1073/pnas.1120310109
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Marine viruses impose a heavy mortality on their host bacteria, whereas at the same time the degree of viral resistance in marine bacteria appears to be high. Antagonistic coevolution-the reciprocal evolutionary change of interacting species-might reconcile these observations, if it leads to rapid and dynamic levels of viral resistance. Here we demonstrate the potential for extensive antagonistic coevolution between the ecologically important marine cyanobacterium Synechococcus and a lytic virus. In a 6-mo-long replicated chemostat experiment, Synechococcus sp. WH7803 and the virus (RIM8) underwent multiple coevolutionary cycles, leading to the rapid diversification of both host and virus. Over the course of the experiment, we detected between 4 and 13 newly evolved viral phenotypes (differing in host range) and between 4 and 11 newly evolved Synechococcus phenotypes (differing in viral resistance) in each chemostat. Genomic analysis of isolates identified several candidate genes in both the host and virus that might influence their interactions. Notably, none of the viral candidates were tail fiber genes, thought to be the primary determinants of host range in tailed bacteriophages, highlighting the difficulty in generalizing results from bacteriophage infecting.-Proteobacteria. Finally, we show that pairwise virus-host coevolution may have broader community consequences; coevolution in the chemostat altered the sensitivity of Synechoccocus to a diverse suite of viruses, as well as the virus' ability to infect additional Synechococcus strains. Our results indicate that rapid coevolution may contribute to the generation and maintenance of Synechococcus and virus diversity and thereby influence viral-mediated mortality of these key marine bacteria.
引用
收藏
页码:4544 / 4549
页数:6
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