Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures

被引：1

作者：

Abashina, Tatiana ^{[1
,2
]}

Vainshtein, Mikhail ^{[1
]}

Korpela, Saara ^{[3
,4
]}

Korpela, Timo ^{[3
]}

机构：

[1] Russ Acad Sci, Inst Biochem Physiol Microorganisms, Prospekt Nauki 5, Pushchino 142290, Russia

[2] Pushchino State Inst Nat Sci, Prospekt Nauki 3, Pushchino 142290, Russia

[3] Univ Turku, Dept Future Technol, FI-20014 Turku, Finland

[4] Labmaster Ltd, Fiskarsinkatu 11, Turku 20610, Finland

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2018年 / 153卷

关键词：

Density gradient; differential centrifugation; separation of cells and particles; solidifying gradients; CENTRIFUGATION;

D O I：

10.1016/j.mimet.2018.09.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Gelatin added in certain concentration into the density gradient mixtures allows to solidify and melt the gradient reversibly. Such a method was applied to separation of partially disintegrated Escherichia coli VKM 13-125 cells in discontinuous sucrose gradient which was optimized to obtain small round "nano-cells" free from other cell components. Gelatin added to the sucrose solutions had the advantages: i) accurate gradient layers were formed, ii) gelatin did not interfere with the cells and sub-particles during centrifugation, iii) solidified gelatin allowed to cut out the separated cell components, and iv) gelatin allowed to prepare ready-made solidified gradient centrifugation tubes into storage.

引用

页码：45 / 47

页数：3

共 11 条

[1] Link between the early calcium deposition in placenta and nanobacterial-like infection [J].