Preparation of samples for comparative studies of arthropod chromosomes:: Visualization, in situ hybridization, and genome size estimation

被引:28
作者
DeSalle, R [1 ]
Gregory, TR
Johnston, JS
机构
[1] Amer Museum Nat Hist, Dept Invertebrate Zool, New York, NY 10024 USA
[2] Nat Hist Museum, Dept Entomol, London SW7 5BD, England
[3] Texas A&M Univ, Dept Entomol, College Stn, TX 77843 USA
来源
MOLECULAR EVOLUTION: PRODUCING THE BIOCHEMICAL DATA, PART B | 2005年 / 395卷
基金
加拿大自然科学与工程研究理事会;
关键词
D O I
10.1016/S0076-6879(05)95025-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The ability to obtain large amounts of genomic sequence for organisms and high throughput technology has led to a change in the thrust of research at the level of chromosomes in animals. In the past chromosomal analysis of animals was focused on gross changes such as inversions, translocations, and deletions for both genetic and evolutionary studies. The advent of in situ hybridization technology and the ability to measure genome content size changed both the precision and the scale of studies addressing chromosomal change as a tool in evolutionary biology. This chapter addresses two of the major areas of change that have occurred in chromosomal studies in the past decade - examination of more refined and genome enabled structural changes in chromosomes and genome size measure. This cahpter describes some of the chromosome structure approaches such as fluorescent in situ hybridization (FISH), comparative genomic hybridization (CGH) and other techniques. As well, advances in Genome size measurement and theory are described herein.
引用
收藏
页码:460 / 488
页数:29
相关论文
共 69 条
[1]  
[Anonymous], PLANT DNA C VALUES D
[2]  
Ashburner M., 1989, DROSOPHILA LAB MANUA
[3]  
Balbiani, 1881, ZOOL ANZ, V4, P637
[4]  
Barch M.J.K.T., 1997, The AGT Cytogenetics Laboratory Manual
[5]   GEOGRAPHICAL PARTHENOGENESIS AND POLYPLOIDY IN DAPHNIA-PULEX [J].
BEATON, MJ ;
HEBERT, PDN .
AMERICAN NATURALIST, 1988, 132 (06) :837-845
[6]   MINIATURE GENOMES AND ENDOPOLYPLOIDY IN CLADOCERAN CRUSTACEANS [J].
BEATON, MJ ;
HEBERT, PDN .
GENOME, 1989, 32 (06) :1048-1053
[7]  
BEATON MJ, 1995, THESIS U GUELPH GUEL
[8]   Comparisons with Caenorhabditis (∼100 Mb) and Drosophila (∼175 Mb) using flow cytometry show genome size in Arabidopsis to be ∼157 Mb and thus ∼25 % larger than the Arabidopsis genome initiative estimate of ∼125 Mb [J].
Bennett, MD ;
Leitch, IJ ;
Price, HJ ;
Johnston, JS .
ANNALS OF BOTANY, 2003, 91 (05) :547-557
[9]   NUCLEAR-DNA AMOUNTS IN ANGIOSPERMS [J].
BENNETT, MD ;
SMITH, JB .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1976, 274 (933) :227-274
[10]  
BLACKMAN RL, 1980, INSECT CYTOGENETICS