A Proton-Detected 4D Solid-State NMR Experiment for Protein Structure Determination

被引：139

作者：

Huber, Matthias ^{[1
]}

Hiller, Sebastian ^{[1
]}

Schanda, Paul ^{[1
]}

Ernst, Matthias ^{[1
]}

Boeckmann, Anja ^{[2
]}

Verel, Rene ^{[1
]}

Meier, Beat H. ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, CH-8093 Zurich, Switzerland

[2] Univ Lyon 1, Inst Biol & Chim Prot, CNRS, UMR 5086, F-69367 Lyon, France

来源：

CHEMPHYSCHEM | 2011年 / 12卷 / 05期

基金：

瑞士国家科学基金会;

关键词：

dipolar recoupling; proteins; protein structures; proton detection; solid-state NMR spectroscopy; ANGLE-SPINNING NMR; MAXIMUM-ENTROPY RECONSTRUCTION; METHYL-GROUPS; MICROCRYSTALLINE UBIQUITIN; BIOLOGICAL MACROMOLECULES; ASSIGNMENT STRATEGIES; ULTRAHIGH-RESOLUTION; AMYLOID FIBRILS; SPECTROSCOPY; SPECTRA;

D O I：

10.1002/cphc.201100062

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Adding a new dimension: 4D or 3D proton-detected spectra of perdeuterated protein samples with 1H labelled amides and methyl groups permit collecting unambiguous distance restraints with high sensitivity and determining protein structure by solid-state NMR (see picture). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：915 / 918

页数：4

共 55 条

[11] A simple and effective NMR cell for studies of encapsulated proteins dissolved in low viscosity solvents [J].

Flynn, PF ;

Milton, MJ ;

Babu, CR ;

Wand, AJ .

JOURNAL OF BIOMOLECULAR NMR, 2002, 23 (04) :311-316

[12]

Gans P., 2010, ANGEW CHEM, V122, P2002, DOI DOI 10.1002/ANGE.200905660

[13] Stereospecific Isotopic Labeling of Methyl Groups for NMR Spectroscopic Studies of High-Molecular-Weight Proteins [J].

Gans, Pierre ;

Hamelin, Olivier ;

Sounier, Remy ;

Ayala, Isabel ;

Dura, M. Asuncion ;

Amero, Carlos D. ;

Noirclerc-Savoye, Marjolaine ;

Franzetti, Bruno ;

Plevin, Michael J. ;

Boisbouvier, Jerome .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2010, 49 (11) :1958-1962

[14] A robust and cost-effective method for the production of Val, Leu, Ile (δ1) methyl-protonated 15N-, 13C-, 2H-labeled proteins [J].

Goto, NK ;

Gardner, KH ;

Mueller, GA ;

Willis, RC ;

Kay, LE .

JOURNAL OF BIOMOLECULAR NMR, 1999, 13 (04) :369-374

[15] Torsion angle dynamics for NMR structure calculation with the new program DYANA [J].

Guntert, P ;

Mumenthaler, C ;

Wuthrich, K .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 273 (01) :283-298

[16] PROCESSING OF MULTIDIMENSIONAL NMR DATA WITH THE NEW SOFTWARE PROSA [J].

GUNTERT, P ;

DOTSCH, V ;

WIDER, G ;

WUTHRICH, K .

JOURNAL OF BIOMOLECULAR NMR, 1992, 2 (06) :619-629

[17] Protein NMR structure determination with automated NOE assignment using the new software CANDID and the torsion angle dynamics algorithm DYANA [J].

Herrmann, T ;

Güntert, P ;

Wüthrich, K .

JOURNAL OF MOLECULAR BIOLOGY, 2002, 319 (01) :209-227

[18] Coupled Decomposition of Four-Dimensional NOESY Spectra [J].

Hiller, Sebastian ;

Ibraghimov, Ilghis ;

Wagner, Gerhard ;

Orekhov, Vladislav Y. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (36) :12970-12978

[19] MAXIMUM-ENTROPY RECONSTRUCTION OF COMPLEX (PHASE-SENSITIVE) SPECTRA [J].

HOCH, JC ;

STERN, AS ;

DONOHO, DL ;

JOHNSTONE, IM .

JOURNAL OF MAGNETIC RESONANCE, 1990, 86 (02) :236-246

[20] Ultrahigh-resolution 1H-13C HSQC spectra of metabolite mixtures using nonlinear sampling and forward maximum entropy reconstruction [J].

Hyberts, Sven G. ;

Heffron, Gregory J. ;

Tarragona, Nestor G. ;

Solanky, Kirty ;

Edmonds, Katherine A. ;

Luithardt, Harry ;

Fejzo, Jasna ;

Chorev, Michael ;

Aktas, Huseyin ;

Colson, Kimberly ;

Falchuk, Kenneth H. ;

Halperin, Jose A. ;

Wagner, Gerhard .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (16) :5108-5116

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