A Proton-Detected 4D Solid-State NMR Experiment for Protein Structure Determination

被引：139

作者：

Huber, Matthias ^{[1
]}

Hiller, Sebastian ^{[1
]}

Schanda, Paul ^{[1
]}

Ernst, Matthias ^{[1
]}

Boeckmann, Anja ^{[2
]}

Verel, Rene ^{[1
]}

Meier, Beat H. ^{[1
]}

机构：

[1] Swiss Fed Inst Technol, CH-8093 Zurich, Switzerland

[2] Univ Lyon 1, Inst Biol & Chim Prot, CNRS, UMR 5086, F-69367 Lyon, France

来源：

CHEMPHYSCHEM | 2011年 / 12卷 / 05期

基金：

瑞士国家科学基金会;

关键词：

dipolar recoupling; proteins; protein structures; proton detection; solid-state NMR spectroscopy; ANGLE-SPINNING NMR; MAXIMUM-ENTROPY RECONSTRUCTION; METHYL-GROUPS; MICROCRYSTALLINE UBIQUITIN; BIOLOGICAL MACROMOLECULES; ASSIGNMENT STRATEGIES; ULTRAHIGH-RESOLUTION; AMYLOID FIBRILS; SPECTROSCOPY; SPECTRA;

D O I：

10.1002/cphc.201100062

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Adding a new dimension: 4D or 3D proton-detected spectra of perdeuterated protein samples with 1H labelled amides and methyl groups permit collecting unambiguous distance restraints with high sensitivity and determining protein structure by solid-state NMR (see picture). Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：915 / 918

页数：4

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