A mutation in the 5′ untranslated region increases stability of norA mRNA, encoding a multidrug resistance transporter of Staphylococcus aureus

被引：53
作者：
Fournier, B
Truong-Bolduc, QC
Zhang, XM
Hooper, DC
机构：
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Div Infect Dis, Boston, MA 02114 USA
[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Med Serv, Boston, MA 02114 USA
来源：
JOURNAL OF BACTERIOLOGY | 2001年 / 183卷 / 07期
关键词：
D O I：
10.1128/JB.183.7.2367-2371.2001
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
NorA, a multidrug efflux pump in Staphylococcus aureus, protects the cell from multiple drugs, including quinolones, The flqB mutation (T-->G) in the 5' untranslated region upstream of norA causes norA overexpression of 4.9-fold in cis, as measured in norA::blaZ fusions. The transcriptional initiation site of norA mas unchanged in mutant and wild-type strains, but the half-life of norA mRNA was increased 4.8-fold in the flqB mutant compared to the wild-type strain. Computer generated folding of the first 68 nucleotides of the norA transcript predicts an additional stem-loop and changes in a putative RNase III cleavage site in the flqB mutant.
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收藏
页码：2367 / 2371
页数：5
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